pET-43.1a(+)
Bacterial vector for expressing NusA fusion proteins. For other reading frames, use pET-43.1b(+) or pET-43.1c(+).
Sequence Author: MilliporeSigma (Novagen)
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Sticky ends from different DraIII sites may not be compatible. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BglI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Sticky ends from different PpuMI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different BsmI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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ApaI can be used between 25°C and 37°C. |
| enterokinase site 680 .. 694 = 15 bp 5 amino acids = 606.5 Da Product: enterokinase recognition and cleavage site |
| enterokinase site 680 .. 694 = 15 bp 5 amino acids = 606.5 Da Product: enterokinase recognition and cleavage site |
| thrombin site 713 .. 730 = 18 bp 6 amino acids = 627.7 Da Product: thrombin recognition and cleavage site |
| thrombin site 713 .. 730 = 18 bp 6 amino acids = 627.7 Da Product: thrombin recognition and cleavage site |
| S-Tag 746 .. 790 = 45 bp 15 amino acids = 1.7 kDa Product: affinity and epitope tag derived from pancreatic ribonuclease A |
| S-Tag 746 .. 790 = 45 bp 15 amino acids = 1.7 kDa Product: affinity and epitope tag derived from pancreatic ribonuclease A |
| 6xHis 800 .. 817 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 800 .. 817 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| NusA 833 .. 2317 = 1485 bp 495 amino acids = 54.9 kDa Product: transcription elongation protein (N utilization substance protein A) highly soluble in E. coli |
| NusA 833 .. 2317 = 1485 bp 495 amino acids = 54.9 kDa Product: transcription elongation protein (N utilization substance protein A) highly soluble in E. coli |
| lacI 2796 .. 3878 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lacI 2796 .. 3878 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| AmpR 5833 .. 6693 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 5833 .. 5901 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5833 .. 6693 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 5902 .. 6693 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5833 .. 6693 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 5071 .. 5659 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 5071 .. 5659 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 6808 .. 7263 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 6808 .. 7263 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| rop 4450 .. 4641 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein |
| rop 4450 .. 4641 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein |
| rrnB T1 terminator 259 .. 345 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
| rrnB T1 terminator 259 .. 345 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
| lacI promoter 2718 .. 2795 = 78 bp |
| lacI promoter 2718 .. 2795 = 78 bp |
| 6xHis 511 .. 528 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 511 .. 528 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| HSV tag 535 .. 567 = 33 bp 11 amino acids = 1.2 kDa Product: HSV (herpes simplex virus) epitope tag |
| HSV tag 535 .. 567 = 33 bp 11 amino acids = 1.2 kDa Product: HSV (herpes simplex virus) epitope tag |
| T7 terminator 25 .. 72 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
| T7 terminator 25 .. 72 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
| lac operator 2362 .. 2386 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lac operator 2362 .. 2386 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| T7 promoter 2387 .. 2405 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| T7 promoter 2387 .. 2405 = 19 bp promoter for bacteriophage T7 RNA polymerase |
| RBS 2326 .. 2331 = 6 bp ribosome binding site |
| RBS 2326 .. 2331 = 6 bp < |