pETcoco-1
Bacterial vector with a chloramphenicol resistance marker that allows single-copy replication, on-demand amplification, and tightly regulated expression.
Sequence Author: MilliporeSigma (Novagen)
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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FseI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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sopA 7457 .. 8632 = 1176 bp 391 amino acids = 44.1 kDa Product: partitioning protein for the bacterial F plasmid |
sopA 7457 .. 8632 = 1176 bp 391 amino acids = 44.1 kDa Product: partitioning protein for the bacterial F plasmid |
trfA 2080 .. 3228 = 1149 bp 382 amino acids = 43.8 kDa Product: trans-acting replication protein that binds to and activates oriV |
trfA 2080 .. 3228 = 1149 bp 382 amino acids = 43.8 kDa Product: trans-acting replication protein that binds to and activates oriV |
lacI 10,985 .. 12,067 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lacI 10,985 .. 12,067 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
araC 831 .. 1709 = 879 bp 292 amino acids = 33.4 kDa Product: L-arabinose regulatory protein |
araC 831 .. 1709 = 879 bp 292 amino acids = 33.4 kDa Product: L-arabinose regulatory protein |
repE 6123 .. 6878 = 756 bp 251 amino acids = 29.4 kDa Product: replication initiation protein for the bacterial F plasmid |
repE 6123 .. 6878 = 756 bp 251 amino acids = 29.4 kDa Product: replication initiation protein for the bacterial F plasmid |
CmR 3396 .. 4055 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
CmR 3396 .. 4055 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
oriV 5099 .. 5713 = 615 bp origin of replication for the bacterial F plasmid |
oriV 5099 .. 5713 = 615 bp origin of replication for the bacterial F plasmid |
sopC 9676 .. 10,149 = 474 bp centromere-like partitioning region of the bacterial F plasmid |
sopC 9676 .. 10,149 = 474 bp centromere-like partitioning region of the bacterial F plasmid |
cos 10,409 .. 10,807 = 399 bp lambda cos site; allows packaging into phage lambda particles |
cos 10,409 .. 10,807 = 399 bp lambda cos site; allows packaging into phage lambda particles |
incC 6881 .. 7131 = 251 bp incompatibility region of the bacterial F plasmid |
incC 6881 .. 7131 = 251 bp incompatibility region of the bacterial F plasmid |
MCS 70 .. 314 = 245 bp multiple cloning site |
MCS< |