pET-32 Ek_LIC
Bacterial vector for ligation-independent cloning (LIC) to express thioredoxin-tagged proteins with an enterokinase site.
Sequence Author: MilliporeSigma (Novagen)
Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets
No matches
|
|
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
| ||
Sticky ends from different BlpI sites may not be compatible. |
| ||
Sticky ends from different DraIII sites may not be compatible. |
|
|
|
|
| ||
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
Sticky ends from different AlwNI sites may not be compatible. |
| ||
PciI is inhibited by nonionic detergents. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
|
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
|
|
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
|
| ||
Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different BstAPI sites may not be compatible. |
|
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
|
| ||
ApaI can be used between 25°C and 37°C. |
| ||
BssHII is typically used at 50°C, but is 75% active at 37°C. |
|
| ||
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
|
| ||
Sticky ends from different PpuMI sites may not be compatible. |
|