pET-41b(+)
Bacterial vector for expressing GST fusion proteins with an enterokinase site. For other reading frames, use pET-41a(+) or pET-41c(+).
Sequence Author: MilliporeSigma (Novagen)
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present. Sticky ends from different AcuI sites may not be compatible.After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different AlwNI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Sticky ends from different BstAPI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different PpuMI sites may not be compatible. |
| lacI 1573 .. 2655 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lacI 1573 .. 2655 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| 8xHis 150 .. 173 = 24 bp 8 amino acids = 1.1 kDa Product: 8xHis affinity tag |
| 8xHis 150 .. 173 = 24 bp 8 amino acids = 1.1 kDa Product: 8xHis affinity tag |
| enterokinase site 264 .. 278 = 15 bp 5 amino acids = 606.5 Da Product: enterokinase recognition and cleavage site |
| enterokinase site 264 .. 278 = 15 bp 5 amino acids = 606.5 Da Product: enterokinase recognition and cleavage site |
| S-Tag 309 .. 353 = 45 bp 15 amino acids = 1.7 kDa Product: affinity and epitope tag derived from pancreatic ribonuclease A |
| S-Tag 309 .. 353 = 45 bp 15 amino acids = 1.7 kDa Product: affinity and epitope tag derived from pancreatic ribonuclease A |
| thrombin site 369 .. 386 = 18 bp 6 amino acids = 627.7 Da Product: thrombin recognition and cleavage site |
| thrombin site 369 .. 386 = 18 bp 6 amino acids = 627.7 Da Product: thrombin recognition and cleavage site |
| 6xHis 396 .. 413 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 396 .. 413 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| GST 441 .. 1094 = 654 bp 218 amino acids = 25.5 kDa Product: glutathione S-transferase from Schistosoma japonicum |
| GST 441 .. 1094 = 654 bp 218 amino acids = 25.5 kDa Product: glutathione S-transferase from Schistosoma japonicum |
| KanR 4558 .. 5373 = 816 bp 271 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 4558 .. 5373 = 816 bp 271 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
| ori 3848 .. 4436 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3848 .. 4436 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 5466 .. 5921 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 5466 .. 5921 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| rop 3227 .. 3418 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein |
| rop 3227 .. 3418 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein |
| lacI promoter 1495 .. 1572 = 78 bp |
| lacI promoter 1495 .. 1572 = 78 bp |
| T7 terminator 26 .. 73 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
| T7 terminator 26 .. 73 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
| lac operator 1139 .. 1163 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lac operator 1139 .. 1163 = 25 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranos |