Bacterial vector with an ampicillin resistance marker that allows single-copy replication, on-demand amplification, and tightly regulated expression.
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PciI is inhibited by nonionic detergents.
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C.
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA.
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
BsrGI is typically used at 37°C, but is even more active at 60°C.
BsiWI is typically used at 55°C, but is 50% active at 37°C.