pETDuet-1

Bacterial vector for the co-expression of two genes.

Sequence Author: MilliporeSigma (Novagen)

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EcoRI (112) BamHI (106) Bsp19I - NcoI (69) RBS XbaI (30) lac operator T7 promoter BanIII * - Bsa29I * - BseCI * - BshVI - BspDI * - BspXI * - Bsu15I * - BsuTUI * - ClaI * (5383) pET Upstream Primer (5358 .. 5373) SgrAI (5339) BbuI - PaeI - SphI (5191) BstENI - EcoNI - XagI (5126) BstAPI (4982) MluI (4658) BclI * - FbaI * - Ksp22I * (4644) BstEII - BstPI - Eco91I - EcoO65I - PspEI (4476) ApaI (4455) Bsp120I - PspOMI (4451) BstHPI - HpaI - KspAI (4156) PpuMI - Psp5II - PspPPI (3789) Bpu10I (3689) AspI - PflFI - PsyI - Tth111I (3052) BssNAI - Bst1107I - BstZ17I (3027) BspQI - LguI - PciSI - SapI (2911) BspLU11I - PciI - PscI (2794) Ecl136II - Eco53kI - EcoICRI (120) Psp124BI - SacI - SstI (122) Acc36I - BfuAI - BspMI - BveI (124) AscI - PalAI - SgsI (125) PstI - SbfI - SdaI - Sse8387I (135) SalI (137) HindIII (143) CciNI - NotI (150) AflII - BfrI - BspTI - Bst98I - BstAFI - MspCI - Vha464I (163) DuetUP2 Primer (189 .. 208) Bsp1407I - BsrGI - BstAUI - SspBI (190) DuetDOWN1 Primer (189 .. 208) T7 promoter RBS FauNDI - NdeI (298) BglII (305) MfeI - MunI (311) Eco32I - EcoRV (319) FseI - RigI (328) AsiSI - RgaI - SfaAI - SgfI (337) ZraI (344) AatII (346) Acc65I - Asp718I (348) KpnI (352) Ama87I - AvaI - BmeT110I - BsiHKCI - BsoBI - Eco88I - PaeR7I - PspXI - Sfr274I - SlaI - XhoI (354) PacI (429) AspA2I - AvrII - BlnI - XmaJI (433) BlpI - Bpu1102I - Bsp1720I - CelII (451) T7 Terminator Primer (448 .. 466) T7 terminator AdeI - DraIII (779) AanI - PsiI (904) SspI (984) AhdI - AspEI - BmeRI - DriI - Eam1105I (1189) BsaI - Bso31I - BspTNI - Eco31I (1250) BglI (1309) Acc16I - AviII - FspI - NsbI (1411) Acc113I - BmcAI - ScaI - ZrmI (1669) AlwNI - CaiI - PstNI (2385)