TAGZyme pQE-1

Vector for bacterial expression of 6xHis-tagged proteins that can be processed by TAGZyme.

Sequence Author: Qiagen

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HomePlasmidsQiagen VectorsTAGZyme pQE-1
AvaI - BsoBI - PaeR7I - XhoI (1) BbsI (3452) EcoO109I (3450) AatII (3396) ZraI (3394) XmnI (3073) PvuI (2844) FspI (2696) AseI (2646) NmeAIII (2622) BglI (2594) BsrFI (2554) BsaI (2535) AhdI (2474) AlwNI (1997) PspFI (1889) BseYI (1885) T5 promoter PsiI (49) MfeI (59) lac operator EcoRI (88) BseRI (115) ATG 6xHis PvuII (141) SphI (148) Eco53kI (154) BanII - SacI (156) EagI - NotI (161) SacII (167) Acc65I (169) KpnI (173) PmlI (177) SalI (180) HincII (182) PstI (190) HindIII (192) BfuAI - BspMI (193) BlpI (204) NheI (312) BmtI (316) Bpu10I (337) BspEI (560) PasI - PflMI * (796) MscI (831) NcoI - StyI (865) XbaI (1168) PfoI (1225) PflFI - Tth111I (1328) BstZ17I (1354) NdeI (1404) BstAPI (1405) BspQI - SapI (1465) PciI (1581) TAGZyme pQE-1 3466 bp
AvaI  (1)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site		 C T C G A G G A G C T C
BbsI  (3452)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (3450)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3396)
1 site		 G A C G T C C T G C A G
ZraI  (3394)
1 site		 G A C G T C C T G C A G
XmnI  (3073)
1 site		 G A A N N N N T T C C T T N N N N A A G
PvuI  (2844)
1 site		 C G A T C G G C T A G C
FspI  (2696)
1 site		 T G C G C A A C G C G T
AseI  (2646)
1 site		 A T T A A T T A A T T A
NmeAIII  (2622)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2594)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BsrFI  (2554)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2535)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2474)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1997)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1889)
1 site		 C C C A G C G G G T C G
BseYI  (1885)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PsiI  (49)
1 site		 T T A T A A A A T A T T
MfeI  (59)
1 site		 C A A T T G G T T A A C
EcoRI  (88)
1 site		 G A A T T C C T T A A G
BseRI  (115)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PvuII  (141)
1 site		 C A G C T G G T C G A C
SphI  (148)
1 site		 G C A T G C C G T A C G
Eco53kI  (154)
1 site		 G A G C T C C T C G A G
BanII  (156)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (156)
1 site		 G A G C T C C T C G A G
EagI  (161)
1 site		 C G G C C G G C C G G C
NotI  (161)
1 site		 G C G G C C G C C G C C G G C G
SacII  (167)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
Acc65I  (169)
1 site		 G G T A C C C C A T G G
KpnI  (173)
1 site		 G G T A C C C C A T G G
PmlI  (177)
1 site		 C A C G T G G T G C A C
SalI  (180)
1 site		 G T C G A C C A G C T G
HincII  (182)
1 site		 G T Y R A C C A R Y T G
PstI  (190)
1 site		 C T G C A G G A C G T C
HindIII  (192)
1 site		 A A G C T T T T C G A A
BfuAI  (193)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (193)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (204)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
NheI  (312)
1 site		 G C T A G C C G A T C G
BmtI  (316)
1 site		 G C T A G C C G A T C G
Bpu10I  (337)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspEI  (560)
1 site		 T C C G G A A G G C C T
PasI  (796)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
PflMI  (796)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
MscI  (831)
1 site		 T G G C C A A C C G G T
NcoI  (865)
1 site		 C C A T G G G G T A C C
StyI  (865)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
XbaI  (1168)
1 site		 T C T A G A A G A T C T
PfoI  (1225)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
PflFI  (1328)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1328)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstZ17I  (1354)
1 site		 G T A T A C C A T A T G
NdeI  (1404)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstAPI  (1405)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BspQI  (1465)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1465)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (1581)
1 site		 A C A T G T