pQE-16
Bacterial vector for expressing DHFR fusion proteins with a C-terminal 6xHis tag. Similar to pQE-19 and pQE-22, but with a different reading frame after the DHFR.
Sequence Author: Qiagen
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BglI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different AvaI sites may not be compatible. |
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Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different PasI sites may not be compatible. |
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Sticky ends from different BtgI sites may not be compatible. |
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Sticky ends from different StyI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Sticky ends from different BstAPI sites may not be compatible. |
AmpR 2931 .. 3791 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 2931 .. 3722 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 2931 .. 3791 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 3723 .. 3791 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 2931 .. 3791 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
CmR 881 .. 1540 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
CmR 881 .. 1540 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
ATG 115 .. 117 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 115 .. 117 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
DHFR 133 .. 690 = 558 bp 186 amino acids = 21.5 kDa Product: mouse dihydrofolate reductase |
DHFR 133 .. 690 = 558 bp 186 amino acids = 21.5 kDa Product: mouse dihydrofolate reductase |
6xHis 703 .. 720 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
6xHis 703 .. 720 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
ori 2172 .. 2760 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 2172 .. 2760 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
AmpR promoter 3792 .. 3896 = 105 bp |
AmpR promoter 3792 .. 3896 = 105 bp |
lambda t0 terminator 743 .. 837 = 95 bp transcription terminator from phage lambda |
lambda t0 terminator 743 .. 837 = 95 bp transcription terminator from phage lambda |
rrnB T1 terminator 1605 .. 1691 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
rrnB T1 terminator 1605 .. 1691 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
T5 promoter 10 .. 54 = 45 bp 4 segments Segment 1: 10 .. 24 = 15 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
T5 promoter 10 .. 54 = 45 bp 4 segments Segment 2: -35 25 .. 30 = 6 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
T5 promoter 10 .. 54 = 45 bp 4 segments Segment 3: 31 .. 47 = 17 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
T5 promoter 10 .. 54 = 45 bp 4 segments Segment 4: -10 48 .. 54 = 7 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
T5 promoter 10 .. 54 = 45 bp 4 segments bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
lac operator 62 .. 78 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 62 .. 78 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
stop codons 727 .. 737 = 11 bp stop codons in all three reading frames |
stop codons 727 .. 737 = 11 bp stop codons in all three reading frames |
RBS 101 .. 106 = 6 bp ribosome binding site |
RBS 101 .. 106 = 6 bp ribosome binding site |
lac operator 30 .. 46 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 30 .. 46 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
ORF: 115 .. 723 = 609 bp ORF: 202 amino acids = 23.3 kDa |
ORF: 3061 .. 3327 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 881 .. 1540 = 660 bp ORF: 219 amino acids = 25.7 kDa |
ORF: 424 .. 717 = 294 bp ORF: 97 amino acids = 11.8 kDa |
ORF: 81 .. 314 = 234 bp ORF: 77 amino acids = 8.8 kDa |
ORF: 2931 .. 3791 = 861 bp ORF: 286 amino acids = 31.6 kDa |
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