pQE-40

Bacterial vector for expressing DHFR fusion proteins with an N-terminal 6xHis tag. For other reading frames, use pQE-41 or pQE-42.

Sequence Author: Qiagen

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BbsI (4017) EcoO109I (4015) AatII (3961) ZraI (3959) FspI (3261) NmeAIII (3187) BglI (3159) BsrFI (3119) AhdI (3039) PspFI (2454) BseYI (2450) PciI (2146) BspQI - SapI (2030) PaeR7I - XhoI (1) PsiI (49) MfeI (59) lac operator EcoRI (88) RBS ATG 6xHis BamHI (145) CsiI - SexAI * (325) Eco53kI (415) BanII - SacI (417) BstXI (435) AflII (447) EcoNI (658) BglII (721) SphI (731) Acc65I (733) KpnI (737) TspMI - XmaI (738) SmaI (740) SalI (743) HincII (745) PstI (753) BfuAI - BspMI (756) HindIII (757) BlpI (769) NheI (877) BmtI (881) PvuII (1029) BspEI (1125) PasI (1361) MscI (1396) BtgI - NcoI - StyI (1430) XbaI (1733) PflFI - Tth111I (1893) BsaAI (1900) BstZ17I (1919) NdeI (1969) BstAPI (1970) pQE-40 4031 bp
BbsI  (4017)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (4015)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3961)
1 site
G A C G T C C T G C A G
ZraI  (3959)
1 site
G A C G T C C T G C A G
FspI  (3261)
1 site
T G C G C A A C G C G T
NmeAIII  (3187)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (3159)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (3119)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AhdI  (3039)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (2454)
1 site
C C C A G C G G G T C G
BseYI  (2450)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI  (2146)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2030)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2030)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BamHI  (145)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
CsiI  (325)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (325)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
Eco53kI  (415)
1 site
G A G C T C C T C G A G
BanII  (417)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (417)
1 site
G A G C T C C