pQE-81L-Kan

Bacterial lacIq vector with a kanamycin resistance marker for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE-80L-Kan or pQE-82L-Kan.

Sequence Author: Qiagen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Qiagen Vectors | More Plasmid Sets
No matches
PsiI (49) PaeR7I - XhoI (1) NruI (4350) BspDI - ClaI (4314) EcoNI (4094) AsiSI - PvuI (4009) XmnI (3618) AcuI (3416) AlwNI (3284) PciI (2868) BspQI - SapI (2752) NdeI (2691) BstZ17I (2641) PflFI - Tth111I (2615) PfoI (2512) FspI - FspAI (2448) PpuMI * (2431) MfeI (59) lac operator EcoRI (88) RBS BseRI (115) ATG 6xHis BamHI (147) SphI (157) Eco53kI (161) SacI (163) Acc65I (165) KpnI (169) SalI (175) PstI (185) BfuAI - BspMI (188) HindIII (189) BlpI (201) NheI (309) BmtI (313) BspEI (557) PasI (793) NcoI - StyI (862) ScaI (978) KasI (1339) NarI * (1340) SfoI (1341) PluTI (1343) HpaI (1476) EcoRV (1532) BssHII (1567) PspOMI (1771) ApaI (1775) NmeAIII (1778) BstEII (1796) BclI * (1964) MluI (1978) pQE-81L-Kan 4543 bp
PsiI  (49)
1 site
T T A T A A A A T A T T
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
NruI  (4350)
1 site
T C G C G A A G C G C T
BspDI  (4314)
1 site
A T C G A T T A G C T A
ClaI  (4314)
1 site
A T C G A T T A G C T A
EcoNI  (4094)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AsiSI  (4009)
1 site
G C G A T C G C C G C T A G C G
PvuI  (4009)
1 site
C G A T C G G C T A G C
XmnI  (3618)
1 site
G A A N N N N T T C C T T N N N N A A G
AcuI  (3416)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (3284)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2868)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2752)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2752)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2691)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2641)
1 site
G T A T A C C A T A T G
PflFI  (2615)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2615)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2512)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspI  (2448)
1 site
T G C G C A A C G C G T
FspAI  (2448)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (2431)
1 site
R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
MfeI  (59)
1 site
C A A T