pQE-81L-Kan

Bacterial lacIq vector with a kanamycin resistance marker for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE-80L-Kan or pQE-82L-Kan.

Sequence Author: Qiagen

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PaeR7I - XhoI (1) NruI (4350) BspDI - ClaI (4314) EcoNI (4094) AsiSI - PvuI (4009) XmnI (3618) AcuI (3416) AlwNI (3284) PciI (2868) BspQI - SapI (2752) NdeI (2691) BstZ17I (2641) PflFI - Tth111I (2615) PfoI (2512) FspI - FspAI (2448) PpuMI * (2431) PsiI (49) MfeI (59) lac operator EcoRI (88) RBS BseRI (115) ATG 6xHis BamHI (147) SphI (157) Eco53kI (161) SacI (163) Acc65I (165) KpnI (169) SalI (175) PstI (185) BfuAI - BspMI (188) HindIII (189) BlpI (201) NheI (309) BmtI (313) BspEI (557) PasI (793) NcoI - StyI (862) ScaI (978) KasI (1339) NarI * (1340) SfoI (1341) PluTI (1343) HpaI (1476) EcoRV (1532) BssHII (1567) PspOMI (1771) ApaI (1775) NmeAIII (1778) BstEII (1796) BclI * (1964) MluI (1978) pQE-81L-Kan 4543 bp
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
NruI  (4350)
1 site
T C G C G A A G C G C T
BspDI  (4314)
1 site
A T C G A T T A G C T A
ClaI  (4314)
1 site
A T C G A T T A G C T A
EcoNI  (4094)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AsiSI  (4009)
1 site
G C G A T C G C C G C T A G C G
PvuI  (4009)
1 site
C G A T C G G C T A G C
XmnI  (3618)
1 site
G A A N N N N T T C C T T N N N N A A G
AcuI  (3416)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (3284)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2868)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2752)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2752)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2691)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2641)
1 site
G T A T A C C A T A T G
PflFI  (2615)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2615)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2512)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspI  (2448)
1 site
T G C G C A A C G C G T
FspAI  (2448)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (2431)
1 site
R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BamHI  (147)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SphI  (157)
1 site
G C A T G C C G T A C G
Eco53kI  (161)
1 site
G A