pQE-82L

Bacterial lacIq vector for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE-80L or pQE-81L.

Sequence Author: Qiagen

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PsiI (49) T5 promoter PaeR7I - XhoI (1) AatII (4682) ZraI (4680) XmnI (4359) PvuI (4130) BglI (3880) BsaI (3821) AhdI (3760) AlwNI (3283) PciI (2867) BspQI - SapI (2751) NdeI (2690) BstZ17I (2640) PflFI - Tth111I (2614) PfoI (2511) FspAI (2447) PpuMI * (2430) MfeI (59) lac operator EcoRI (88) RBS BseRI (115) ATG 6xHis BamHI (146) SphI (156) Eco53kI (160) SacI (162) Acc65I (164) KpnI (168) TspMI - XmaI (169) SmaI (171) SalI (174) PstI (184) BfuAI - BspMI (187) HindIII (188) BlpI (200) NheI (308) BmtI (312) Bpu10I (333) BspEI (556) PasI (792) KasI (1338) NarI * (1339) SfoI (1340) PluTI (1342) HpaI (1475) EcoRV (1531) BssHII (1566) PspOMI (1770) ApaI (1774) BstEII (1795) BclI * (1963) MluI (1977) pQE-82L 4752 bp
PsiI  (49)
1 site
T T A T A A A A T A T T
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
AatII  (4682)
1 site
G A C G T C C T G C A G
ZraI  (4680)
1 site
G A C G T C C T G C A G
XmnI  (4359)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (4130)
1 site
C G A T C G G C T A G C
BglI  (3880)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3821)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3760)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3283)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2867)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2751)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2751)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2690)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2640)
1 site
G T A T A C C A T A T G
PflFI  (2614)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2614)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2511)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspAI  (2447)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (2430)
1 site
R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BamHI  (146)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SphI  (156)
1 site
G C A T G C C G T A C G
Eco53kI  (160)
1 site
G A G C T C C T C G A G
SacI  (162)
1 site
G A G C T C C T C G A G
Acc65I  (164)
1 site
G G T A C C C C A T G G
KpnI  (168)
1 site
G G T A C C C C A T G G