pQE-42
Bacterial vector for expressing DHFR fusion proteins with an N-terminal 6xHis tag. For other reading frames, use pQE-40 or pQE-41.
Sequence Author: Qiagen
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BglI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Sticky ends from different BanII sites may not be compatible. |
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