pQE-81L

Bacterial lacIq vector for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE-80L or pQE-82L.

Sequence Author: Qiagen

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T5 promoter PaeR7I - XhoI (1) AatII (4683) ZraI (4681) XmnI (4360) PvuI (4131) BglI (3881) BsaI (3822) AhdI (3761) AlwNI (3284) PciI (2868) BspQI - SapI (2752) NdeI (2691) BstZ17I (2641) PflFI - Tth111I (2615) PfoI (2512) FspAI (2448) PpuMI * (2431) PsiI (49) MfeI (59) lac operator EcoRI (88) RBS BseRI (115) ATG 6xHis BamHI (147) SphI (157) Eco53kI (161) SacI (163) Acc65I (165) KpnI (169) TspMI - XmaI (170) SmaI (172) SalI (175) PstI (185) BfuAI - BspMI (188) HindIII (189) BlpI (201) NheI (309) BmtI (313) Bpu10I (334) BspEI (557) PasI (793) NcoI - StyI (862) KasI (1339) NarI * (1340) SfoI (1341) PluTI (1343) HpaI (1476) EcoRV (1532) BssHII (1567) PspOMI (1771) ApaI (1775) BstEII (1796) BclI * (1964) MluI (1978) pQE-81L 4753 bp
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
AatII  (4683)
1 site
G A C G T C C T G C A G
ZraI  (4681)
1 site
G A C G T C C T G C A G
XmnI  (4360)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (4131)
1 site
C G A T C G G C T A G C
BglI  (3881)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3822)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3761)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3284)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2868)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2752)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2752)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2691)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2641)
1 site
G T A T A C C A T A T G
PflFI  (2615)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2615)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2512)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspAI  (2448)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (2431)
1 site
R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BamHI  (147)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SphI  (157)
1 site
G C A T G C C G T A C G
Eco53kI  (161)
1 site
G A G C T C C T C G A G
SacI  (163)
1 site
G A G C T C C T C G A G
Acc65I  (165)
1 site
G G T A C C C C A T G G
KpnI  (169)
1 site
G G T A C C C C A T G G
TspMI  (170)
1 site
C C C G G G G G G C C C
XmaI  (170)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (172)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SalI  (175)
1 site
G T