pQE-80L

Bacterial lacIq vector for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE-81L or pQE-82L.

Sequence Author: Qiagen

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PsiI (49) T5 promoter PaeR7I - XhoI (1) AatII (4681) ZraI (4679) XmnI (4358) PvuI (4129) BglI (3879) BsaI (3820) AhdI (3759) AlwNI (3282) PciI (2866) BspQI - SapI (2750) NdeI (2689) BstZ17I (2639) PflFI - Tth111I (2613) PfoI (2510) FspAI (2446) PpuMI * (2429) MfeI (59) lac operator EcoRI (88) RBS BseRI (115) ATG 6xHis BamHI (145) SphI (155) Eco53kI (159) SacI (161) Acc65I (163) KpnI (167) TspMI - XmaI (168) SmaI (170) SalI (173) PstI (183) BfuAI - BspMI (186) HindIII (187) BlpI (199) NheI (307) BmtI (311) Bpu10I (332) BspEI (555) PasI (791) NcoI - StyI (860) KasI (1337) NarI * (1338) SfoI (1339) PluTI (1341) HpaI (1474) EcoRV (1530) BssHII (1565) PspOMI (1769) ApaI (1773) BstEII (1794) BclI * (1962) MluI (1976) pQE-80L 4751 bp
PsiI  (49)
1 site
T T A T A A A A T A T T
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
AatII  (4681)
1 site
G A C G T C C T G C A G
ZraI  (4679)
1 site
G A C G T C C T G C A G
XmnI  (4358)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (4129)
1 site
C G A T C G G C T A G C
BglI  (3879)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3820)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3759)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3282)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2866)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2750)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2750)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2689)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2639)
1 site
G T A T A C C A T A T G
PflFI  (2613)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2613)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2510)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspAI  (2446)
1 site
R T G C G C A Y Y A C G C G T R
PpuMI  (2429)
1 site
R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BamHI  (145)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SphI  (155)
1 site
G C A T G C C G