pQE-30 UA
Bacterial expression and 6xHis-tagging vector for UA cloning of a PCR product.
Sequence Author: Qiagen
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Sticky ends from different BglI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Sticky ends from different BanII sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Sticky ends from different BlpI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Sticky ends from different PasI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different PflMI sites may not be compatible. |
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Sticky ends from different BtgI sites may not be compatible. |
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Sticky ends from different StyI sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Sticky ends from different BstAPI sites may not be compatible. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
| AmpR 2439 .. 3299 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 2439 .. 3230 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 2439 .. 3299 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 3231 .. 3299 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 2439 .. 3299 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| CmR 389 .. 1048 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 389 .. 1048 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| ori 1680 .. 2268 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 1680 .. 2268 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| AmpR promoter 3300 .. 3404 = 105 bp |
| AmpR promoter 3300 .. 3404 = 105 bp |
| lambda t0 terminator 251 .. 345 = 95 bp transcription terminator from phage lambda |
| lambda t0 terminator 251 .. 345 = 95 bp transcription terminator from phage lambda |
| MCS 145 .. 235 = 91 bp multiple cloning site |
| MCS 145 .. 235 = 91 bp multiple cloning site |
| rrnB T1 terminator 1113 .. 1199 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
| rrnB T1 terminator 1113 .. 1199 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
| T5 promoter 10 .. 54 = 45 bp 4 segments Segment 1: 10 .. 24 = 15 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
| T5 promoter 10 .. 54 = 45 bp 4 segments Segment 2: -35 25 .. 30 = 6 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
| T5 promoter 10 .. 54 = 45 bp 4 segments Segment 3: 31 .. 47 = 17 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
| T5 promoter 10 .. 54 = 45 bp 4 segments Segment 4: -10 48 .. 54 = 7 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
| T5 promoter 10 .. 54 = 45 bp 4 segments bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator |
| ATG 115 .. 117 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| ATG 115 .. 117 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
| 6xHis 127 .. 144 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| 6xHis 127 .. 144 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
| lac operator 62 .. 78 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lac operator 62 .. 78 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| RBS 101 .. 106 = 6 bp |