pDrive
Bacterial vector for UA cloning and expression of a PCR product.
Sequence Author: Qiagen
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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PciI is inhibited by nonionic detergents. |
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After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different PflMI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different StyI sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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BsaHI is typically used at 37°C, but is even more active at 60°C. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
AmpR 1175 .. 2035 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 1175 .. 1243 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 1175 .. 2035 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 1244 .. 2035 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 1175 .. 2035 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
KanR 2181 .. 2996 = 816 bp 271 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
KanR 2181 .. 2996 = 816 bp 271 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
ori 3081 .. 3669 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 3081 .. 3669 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 588 .. 1043 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 588 .. 1043 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
MCS 266 .. 393 = 128 bp multiple cloning site |
MCS 266 .. 393 = 128 bp multiple cloning site |
AmpR promoter 1070 .. 1174 = 105 bp |
AmpR promoter 1070 .. 1174 = 105 bp |
lac promoter 142 .. 172 = 31 bp 3 segments Segment 1: -35 142 .. 147 = 6 bp promoter for the E. coli lac operon |
lac promoter 142 .. 172 = 31 bp 3 segments Segment 2: 148 .. 165 = 18 bp promoter for the E. coli lac operon |
lac promoter 142 .. 172 = 31 bp 3 segments Segment 3: -10 166 .. 172 = 7 bp promoter for the E. coli lac operon |
lac promoter 142 .. 172 = 31 bp 3 segments promoter for the E. coli lac operon |
T7 promoter 239 .. 257 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 239 .. 257 = 19 bp promoter for bacteriophage T7 RNA polymerase |
SP6 promoter 399 .. 417 = 19 bp promoter for bacteriophage SP6 RNA polymerase |
SP6 promoter 399 .. 417 = 19 bp promoter for bacteriophage SP6 RNA polymerase |
lac operator 180 .. 196 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 180 .. 196 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
M13 rev 204 .. 220 = 17 bp common sequencing primer, one of multiple similar variants |
M13 rev 204 .. 220 = 17 bp common sequencing primer, one of multiple similar variants |
M13 fwd 431 .. 447 = 17 bp common sequencing primer, one of multiple similar variants |
M13 fwd 431 .. 447 = 17 bp common sequencing primer, one of multiple similar variants |
lacZα 216 .. 596 = 381 bp 126 amino acids = 14.5 kDa Product: LacZα fragment of β-galactosidase |
lacZα 216 .. 596 = 381 bp 126 amino acids = 14.5 kDa Product: LacZα fragment of β-galactosidase |
ORF: 1175 .. 2035 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 216 .. 596 = 381 bp ORF: 126 amino acids = 14.5 kDa |
ORF: 2181 .. 2996 = 816 bp ORF: 271 amino acids = 31.0 kDa |
ORF: 1639 .. 1905 = 267 bp ORF: 88 amino acids = 9.2 kDa |
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