pIX 4.0

Eukaryotic in vitro translation vector for cloning genes to be expressed using the EasyXpress Insect Kit.

Sequence Author: Qiagen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Qiagen Vectors | More Plasmid Sets
No matches
ZraI (3077) SspI (2961) XmnI (2756) ScaI (2637) TsoI (2556) NmeAIII (2305) BpmI (2227) AhdI (2157) AlwNI (1680) PspFI (1572) AatII (3079) PfoI (46) NdeI (184) BstAPI (185) HindIII (399) SphI (409) AfeI (475) SgrAI (557) NgoMIV (566) NaeI (568) BsaBI * (597) XbaI (624) NcoI (681) BseRI (685) PaeR7I - PspXI - XhoI (697) EagI - NotI (703) BglII - PstI (714) SpeI (736) BamHI (772) BlpI (825) EcoRV (885) TspMI - XmaI (888) SmaI (890) SwaI (897) StuI (904) ApoI - EcoRI (908) M13 rev lac operator lac promoter BspQI - SapI (1148) AflIII - PciI (1264) BseYI (1568) pIX 4.0 3144 bp
ZraI  (3077)
1 site
G A C G T C C T G C A G
SspI  (2961)
1 site
A A T A T T T T A T A A
XmnI  (2756)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2637)
1 site
A G T A C T T C A T G A
TsoI  (2556)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (2305)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (2227)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AhdI  (2157)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1680)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1572)
1 site
C C C A G C G G G T C G
AatII  (3079)
1 site
G A C G T C C T G C A G
PfoI  (46)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
NdeI  (184)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstAPI  (185)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
HindIII  (399)
1 site
A A G C T T T T C G A A
SphI  (409)
1 site
G C A T G C C G T A C G
AfeI  (475)
1 site
A G C G C T T C G C G A
SgrAI  (557)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
NgoMIV  (566)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (568)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsaBI  (597)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
XbaI  (624)
1 site
T C T A G A A G A T C T
NcoI  (681)
1 site
C C A T G G G G T A C C
BseRI  (685)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PaeR7I  (697)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (697)
1 site
V C T C G A G B B G A G C T C V
XhoI  (697)
1 site
C T C G A G G A G C T C
EagI  (703)
1 site
C G G C C G G C C G G C
NotI  (703)
1 site
G C G G C C G C C G C C G G C G
BglII  (714)
1 site
A G A T C T T C T A G A
PstI  (714)
1 site
C T G C A G G A C G T C
SpeI  (736)
1 site
A C T A G T T G A T C A
BamHI  (772)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BlpI  (825)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoRV  (885)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (888)
1 site
C C C G G G G G G C C C
XmaI  (888)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (890)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SwaI  (897)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
StuI  (904)
1 site
A G G C C T T C C G G A
ApoI  (908)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (908)
1 site
G A A T T C C T T A A G
BspQI  (1148)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1148)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (1264)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (1264)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BseYI  (1568)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AmpR
2084 .. 2944  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2084 .. 2875  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2084 .. 2944  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2876 .. 2944  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2084 .. 2944  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1325 .. 1913  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1325 .. 1913  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
2945 .. 3049  =  105 bp
AmpR promoter
2945 .. 3049  =  105 bp
MCS
681 .. 741  =  61 bp
multiple cloning site
MCS
681 .. 741  =  61 bp
multiple cloning site
T7 terminator
836 .. 883  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
836 .. 883  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac promoter
971 .. 1001  =  31 bp
3 segments
   Segment 3:  -10  
   971 .. 977  =  7 bp
promoter for the E. coli lac operon
lac promoter
971 .. 1001  =  31 bp
3 segments
   Segment 2:  
   978 .. 995  =  18 bp
promoter for the E. coli lac operon
lac promoter
971 .. 1001  =  31 bp
3 segments
   Segment 1:  -35  
   996 .. 1001  =  6 bp
promoter for the E. coli lac operon
lac promoter
971 .. 1001  =  31 bp
3 segments
promoter for the E. coli lac operon
T7 promoter
604 .. 622  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
604 .. 622  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
923 .. 939  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
923 .. 939  =  17 bp
common sequencing primer, one of multiple similar variants
lac operator
947 .. 963  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
947 .. 963  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
linearization sites
883 .. 907  =  25 bp
blunt cutter sites for linearizing the vector for in vitro transcription
linearization sites
883 .. 907  =  25 bp
blunt cutter sites for linearizing the vector for in vitro transcription
stop codons
719 .. 732  =  14 bp
stop codons in all three reading frames
stop codons
719 .. 732  =  14 bp
stop codons in all three reading frames
ATG
683 .. 685  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
683 .. 685  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ORF:  186 .. 533  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  2214 .. 2480  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  146 .. 481  =  336 bp
ORF:  111 amino acids  =  12.2 kDa
ORF:  2084 .. 2944  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pIX 4.0.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.