pAB-bee

Baculovirus transfer plasmid for high-level expression of secreted proteins with a C-terminal 8xHis tag.

Sequence Author: AB Vector

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NdeI (9718) ScaI (9027) AlwNI (8070) PspFI (7962) BseYI (7958) BspQI - SapI (7538) AanI (6910) FspAI (6621) SgrDI (6468) AanI (6279) SgrAI (6199) AanI (5136) SnaBI (5111) AanI (121) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) PaeR7I - PspXI - XhoI (1901) SphI (2135) BclI * (2232) AanI (3309) AanI (3398) BbvCI - Bpu10I (3405) NgoMIV (3770) NaeI (3772) EcoRV (4000) BglII (4134) BstZ17I (4184) EagI - NotI (4204) EcoRI (4215) XbaI (4219) TspMI - XmaI (4230) SmaI (4232) BamHI (4234) EcoRI (4406) AvrII (4455) PpuMI - SanDI (4480) pAB-beeā„¢ 9932 bp
NdeI  (9718)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
ScaI  (9027)
1 site
A G T A C T T C A T G A
AlwNI  (8070)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7962)
1 site
C C C A G C G G G T C G
BseYI  (7958)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (7538)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7538)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AanI  (6910)
6 sites
T T A T A A A A T A T T
FspAI  (6621)
1 site
R T G C G C A Y Y A C G C G T R
SgrDI  (6468)
1 site
C G T C G A C G G C A G C T G C
AanI  (6279)
6 sites
T T A T A A A A T A T T
SgrAI  (6199)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AanI  (5136)
6 sites
T T A T A A A A T A T T
SnaBI  (5111)
1 site
T A C G T A A T G C A T
AanI  (121)
6 sites