pBAC-2cp

Baculovirus transfer plasmid with the polyhedrin promoter, encoding an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette.

Sequence Author: MilliporeSigma (Novagen)

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DraIII (5119) ScaI (4447) NmeAIII (4115) BpmI (4037) BmrI (4007) AlwNI (3490) PspFI (3382) BseYI (3378) PciI (3074) PmeI (2954) SgrAI (2666) BsmI (85) BstXI (315) NsiI (332) MluI (547) BbvCI - Bpu10I (564) BglII (1161) BsaBI (1192) NcoI (1264) ATG SacII (1294) thrombin site PflMI (1355) NheI (1365) BmtI (1369) enterokinase site BseRI - TspMI - XmaI (1397) SmaI - SrfI (1399) StuI * (1417) BamHI (1421) EcoRI (1427) Eco53kI (1435) SacI (1437) HindIII (1440) EagI - NotI (1447) PaeR7I - PspXI - XhoI (1455) 6xHis AvrII (1482) BlpI (1491) SphI (1501) SnaBI (1578) SwaI (1668) ZraI (1857) AatII (1859) BmgBI (2082) AgeI (2426) AleI (2657) pBACā„¢-2cp 5411 bp
DraIII  (5119)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (4447)
1 site
A G T A C T T C A T G A
NmeAIII  (4115)
1 site