pTriEx-1.1 Neo

Insect, bacterial, and mammalian vector with a neomycin resistance marker, for expressing proteins with a C-terminal HSV-8xHis cassette.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidsInsect Cell VectorspTriEx-1.1 Neo
ScaI (6295) BpmI (5885) AsiSI (4937) SwaI (4481) SphI (4225) BstAPI (4152) BspHI (3991) BspDI * - ClaI * (3955) PflFI - Tth111I (3358) MluI (399) BbvCI - Bpu10I (416) FseI (1017) SacII (1965) XbaI (2034) PpuMI (2208) PacI (2280) NcoI (2331) EcoRV (2340) TspMI - XmaI (2343) SmaI (2345) Eco53kI (2350) SacI (2352) BamHI (2356) EcoRI (2362) BglII (2370) AscI (2378) SbfI (2388) AgeI (2399) BstBI (2405) NotI (2415) PvuII (2425) BstZ17I (2430) PaeR7I - XhoI (2477) Bsu36I (2519) AvrII (2645) PaqCI (2833) PflMI (2947) KasI (3239) NarI (3240) SfoI (3241) PluTI (3243) MscI (3322) pTriEx™-1.1 Neo 6664 bp
ScaI  (6295)
1 site		 A G T A C T T C A T G A
BpmI  (5885)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AsiSI  (4937)
1 site		 G C G A T C G C C G C T A G C G
SwaI  (4481)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
SphI  (4225)
1 site		 G C A T G C C G T A C G
BstAPI  (4152)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BspHI  (3991)
1 site		 T C A T G A A G T A C T
BspDI  (3955)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3955)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
PflFI  (3358)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3358)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MluI  (399)
1 site		 A C G C G T T G C G C A
BbvCI  (416)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (416)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
FseI  (1017)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
SacII  (1965)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
XbaI  (2034)
1 site		 T C T A G A A G A T C T
PpuMI  (2208)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
PacI  (2280)
1 site		 T T A A T T A A A A T T A A T T
NcoI  (2331)
1 site		 C C A T G G G G T A C C
EcoRV  (2340)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (2343)
1 site		 C C C G G G G G G C C C
XmaI  (2343)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2345)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
Eco53kI  (2350)
1 site		 G A G C T C C T C G A G
SacI  (2352)
1 site		 G A G C T C C T C G A G
BamHI  (2356)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2362)
1 site		 G A A T T C C T T A A G
BglII  (2370)
1 site		 A G A T C T T C T A G A
AscI  (2378)
1 site		 G G C G C G C C C C G C G C G G
SbfI  (2388)
1 site		 C C T G C A G G G G A C G T C C
AgeI  (2399)
1 site		 A C C G G T T G G C C A
BstBI  (2405)
1 site		 T T C G A A A A G C T T
NotI  (2415)
1 site		 G C G G C C G C C G C C G G C G
PvuII  (2425)
1 site		 C A G C T G G T C G A C
BstZ17I  (2430)
1 site		 G T A T A C C A T A T G
PaeR7I  (2477)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2477)
1 site		 C T C G A G G A G C T C
Bsu36I  (2519)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
AvrII  (2645)
1 site		 C C T A G G G G A T C C
PaqCI  (2833)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
PflMI  (2947)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
KasI  (3239)
1 site		 G G C G C C C C G C G G
NarI  (3240)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (3241)
1 site		 G G C G C C C C G C G G
PluTI  (3243)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
MscI  (3322)
1 site		 T G G C C A A C C G G T
Exon 1
1727 .. 1819  =  93 bp
Exon 1
1727 .. 1819  =  93 bp
Intron
1820 .. 2081  =  262 bp
Intron
1820 .. 2081  =  262 bp
Exon 2
2082 .. 4048  =  1967 bp
Exon 2
2082 .. 4048  =  1967 bp
AmpR
5742 .. 6602  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5742 .. 6533  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5742 .. 6602  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6534 .. 6602  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5742 .. 6602  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
4227 .. 4932  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
4227 .. 4932  =  706 bp
contains part of ORF1629
ori
5142 .. 5729  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5142 .. 5729  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
1140 .. 1443  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1140 .. 1443  =  304 bp
human cytomegalovirus immediate early enhancer
chicken β-actin promoter
1449 .. 1726  =  278 bp
chicken β-actin promoter
1449 .. 1726  =  278 bp
T7 terminator
4167 .. 4214  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
4167 .. 4214  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
NeoR/KanR
3103 .. 3906  =  804 bp
267 amino acids  =  29.2 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
3103 .. 3906  =  804 bp
267 amino acids  =  29.2 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
4344 .. 4932  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
4344 .. 4932  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
IRES
2605 .. 3067  =  463 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
2605 .. 3067  =  463 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
ATG
2333 .. 2335  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2333 .. 2335  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
HSV tag
2444 .. 2476  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
HSV tag
2444 .. 2476  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
8xHis
2483 .. 2506  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
2483 .. 2506  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
4046 .. 4157  =  112 bp
rabbit globin terminator
4046 .. 4157  =  112 bp
p10 promoter
2209 .. 2318  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
2209 .. 2318  =  110 bp
baculovirus promoter for expression in insect cells
lac operator
2169 .. 2193  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2169 .. 2193  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
2150 .. 2168  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2150 .. 2168  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
2320 .. 2325  =  6 bp
ribosome binding site
RBS
2320 .. 2325  =  6 bp
ribosome binding site
poly(A) signal
4024 .. 4029  =  6 bp
poly(A) signal
4024 .. 4029  =  6 bp
MCS
2331 .. 2438  =  108 bp
multiple cloning site
MCS
2331 .. 2438  =  108 bp
multiple cloning site
ORF:  1315 .. 1704  =  390 bp
ORF:  129 amino acids  =  13.9 kDa
ORF:  2977 .. 3906  =  930 bp
ORF:  309 amino acids  =  33.9 kDa
ORF:  5872 .. 6138  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  6652 .. 305  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  1547 .. 1798  =  252 bp
ORF:  83 amino acids  =  8.7 kDa
ORF:  3284 .. 3670  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  343 .. 948  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  3421 .. 3675  =  255 bp
ORF:  84 amino acids  =  9.7 kDa
ORF:  387 .. 617  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  1671 .. 2060  =  390 bp
ORF:  129 amino acids  =  13.3 kDa
ORF:  4344 .. 4961  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  5742 .. 6602  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

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TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
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Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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