pIEx Bac-5

Insect cell and baculovirus vector encoding a cleavable N-terminal GST tag and a cleavable C-terminal Strep-Tag® II.

Sequence Author: MilliporeSigma (Novagen)

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DraIII (7207) NmeAIII (6203) BpmI (6125) AlwNI (5578) PspFI (5470) BseYI (5466) PmeI (5042) SgrAI (4754) AleI (4745) AgeI (4514) BmgBI (4170) AatII (3947) ZraI (3945) BstXI (315) NsiI (332) BbvCI - Bpu10I (564) baculovirus recombination region (lef2/ORF603) BglII (1161) EcoRV (1434) NheI (1801) BmtI (1805) PvuII (1971) IE1 promoter BsiWI (1979) PfoI (2278) BspEI (2395) BtgI - NcoI (2433) BspQI - SapI (2514) MscI (2642) KflI (3137) AvaI - BsoBI - TspMI - XmaI (3140) SmaI (3142) Acc65I (3144) KpnI (3148) NotI (3171) Eco53kI (3181) SacI (3183) Strep-Tag II AvrII (3255) StuI (3260) Bsu36I (3262) PflFI - Tth111I (3515) BlpI (3579) SnaBI (3666) pIEx/Bac™-5 7499 bp