pIEx Bac-3 3C_LIC (linearized)

Linearized insect cell and baculovirus vector for ligation-independent cloning (LIC), encoding a cleavable N-terminal 10xHis tag and a cleavable C-terminal Strep-Tag® II.

Sequence Author: MilliporeSigma (Novagen)

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DraIII (4010) BanII (3936) ScaI (3338) NmeAIII (3006) BpmI (2928) AlwNI (2381) PspFI (2273) BseYI (2269) PciI (1965) PmeI (1845) SgrAI (1557) AgeI (1317) BmgBI (973) AatII (750) BstXI (4617) NsiI (4634) BbvCI - Bpu10I (4866) BglII (5463) EcoRV (5736) hr5 enhancer NheI (6103) BmtI (6107) PvuII (6273) BsiWI (6281) EagI (6339) PfoI (6580) EcoO109I - PpuMI (6584) NcoI (6694) ATG 10xHis End (6752) Start (0) thrombin site BstBI (45) Bsu36I (65) IE1 terminator PflFI - Tth111I (318) BlpI (382) SnaBI (469) SwaI (559) ZraI (748) pIEx/Bac™-3 3C/LIC 6752 bp
DraIII  (4010)
1 site
C A C N