pIEx Bac-3 3C_LIC (linearized)

Linearized insect cell and baculovirus vector for ligation-independent cloning (LIC), encoding a cleavable N-terminal 10xHis tag and a cleavable C-terminal Strep-Tag® II.

Sequence Author: MilliporeSigma (Novagen)

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DraIII (4010) BanII (3936) ScaI (3338) NmeAIII (3006) BpmI (2928) AlwNI (2381) PspFI (2273) BseYI (2269) PciI (1965) PmeI (1845) SgrAI (1557) AgeI (1317) BmgBI (973) AatII (750) ZraI (748) BstXI (4617) NsiI (4634) BbvCI - Bpu10I (4866) BglII (5463) EcoRV (5736) NheI (6103) BmtI (6107) PvuII (6273) BsiWI (6281) EagI (6339) PfoI (6580) EcoO109I - PpuMI (6584) NcoI (6694) ATG 10xHis End (6752) Start (0) thrombin site BstBI (45) Bsu36I (65) PflFI - Tth111I (318) BlpI (382) SnaBI (469) SwaI (559) pIEx/Bac™-3 3C/LIC 6752 bp