pBiEx-1
Vector for high-level expression in both bacterial and insect cells of proteins with a cleavable N-terminal 6xHis-S-Tag cassette plus a C-terminal HSV tag.
Sequence Author: MilliporeSigma (Novagen)
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
| ||
PciI is inhibited by nonionic detergents. |
| ||
Sticky ends from different DrdI sites may not be compatible. |
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
|
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different PfoI sites may not be compatible. |
| ||
Sticky ends from different BstAPI sites may not be compatible. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
| * Blocked by Dam methylation. |
| ||
| * Blocked by Dam methylation. |
|
|
|
|
| ||
Sticky ends from different BsmI sites may not be compatible. |
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
| ||
BsiWI is typically used at 55°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the SacII recognition sequence. |
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
|