pTriEx-5 Ek_LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-enterokinase and a C-terminal 10xHis.

Sequence Author: MilliporeSigma (Novagen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspTriEx-5 Ek_LIC (linearized)
Bsu36I (67) NcoI (4961) BstBI (4988) End (5004) Start (0) DraIII (57) pTriEx™-5 Ek/LIC 5004 bp
Bsu36I  (67)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4961)
1 site		 C C A T G G G G T A C C
BstBI  (4988)
1 site		 T T C G A A A A G C T T
End  (5004)
0 sites
Start  (0)
0 sites
DraIII  (57)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1927 .. 2718  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2719 .. 2787  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
ori
1327 .. 1914  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1327 .. 1914  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
Exon 1
4446 .. 4547  =  102 bp
Exon 1
4446 .. 4547  =  102 bp
intron
4548 .. 4711  =  164 bp
intron
4548 .. 4711  =  164 bp
Exon 2
4712 .. 5016  =  305 bp
Exon 2
4712 .. 5016  =  305 bp
CMV enhancer
3925 .. 4228  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3925 .. 4228  =  304 bp
human cytomegalovirus immediate early enhancer
Exon 2
1 .. 233  =  233 bp
Exon 2
1 .. 233  =  233 bp
CMV promoter
4230 .. 4429  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4230 .. 4429  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
T7 terminator
352 .. 399  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
352 .. 399  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
3192 .. 3797  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3192 .. 3797  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
529 .. 1117  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
529 .. 1117  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3030 .. 3154  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3030 .. 3154  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
231 .. 342  =  112 bp
rabbit globin terminator
231 .. 342  =  112 bp
p10 promoter
4839 .. 4948  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
4839 .. 4948  =  110 bp
baculovirus promoter for expression in insect cells
ATG
4963 .. 4965  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4963 .. 4965  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
4972 .. 4995  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
Strep-Tag II
4972 .. 4995  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
enterokinase site
5002 .. 5016  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
5002 .. 5016  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lac operator
4799 .. 4823  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4799 .. 4823  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
4780 .. 4798  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
4780 .. 4798  =  19 bp
promoter for bacteriophage T7 RNA polymerase
poly(A) signal
209 .. 214  =  6 bp
poly(A) signal
209 .. 214  =  6 bp
RBS
4950 .. 4955  =  6 bp
ribosome binding site
RBS
4950 .. 4955  =  6 bp
ribosome binding site
ORF:  2057 .. 2323  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2837 .. 3154  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  529 .. 1146  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  1927 .. 2787  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3192 .. 3797  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  3236 .. 3466  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  4436 .. 4690  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
Click here to try SnapGene

Download pTriEx-5 Ek_LIC (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free