pTriEx-5 Ek_LIC (linearized)
Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-enterokinase and a C-terminal 10xHis.
Sequence Author: MilliporeSigma (Novagen)
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
| ||
Sticky ends from different Bsu36I sites may not be compatible. |
|
|
|
|
| ||
Sticky ends from different DraIII sites may not be compatible. |
AmpR 1927 .. 2787 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 1927 .. 2718 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 1927 .. 2787 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 2719 .. 2787 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 1927 .. 2787 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
baculovirus recombination region (lef2/ORF603) 3030 .. 3857 = 828 bp contains ORF603 and part of lef2 |
baculovirus recombination region (lef2/ORF603) 3030 .. 3857 = 828 bp contains ORF603 and part of lef2 |
baculovirus recombination region (ORF1629) 412 .. 1117 = 706 bp contains part of ORF1629 |
baculovirus recombination region (ORF1629) 412 .. 1117 = 706 bp contains part of ORF1629 |
ori 1327 .. 1914 = 588 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 1327 .. 1914 = 588 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
Exon 1 4446 .. 4547 = 102 bp |
Exon 1 4446 .. 4547 = 102 bp |
intron 4548 .. 4711 = 164 bp |
intron 4548 .. 4711 = 164 bp |
Exon 2 4712 .. 5016 = 305 bp |
Exon 2 4712 .. 5016 = 305 bp |
CMV enhancer 3925 .. 4228 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 3925 .. 4228 = 304 bp human cytomegalovirus immediate early enhancer |
Exon 2 1 .. 233 = 233 bp |
Exon 2 1 .. 233 = 233 bp |
CMV promoter 4230 .. 4429 = 200 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 4230 .. 4429 = 200 bp human cytomegalovirus (CMV) immediate early promoter |
T7 terminator 352 .. 399 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 terminator 352 .. 399 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
ORF603 3192 .. 3797 = 606 bp 201 amino acids = 23.6 kDa Product: baculovirus ORF603 protein |
ORF603 3192 .. 3797 = 606 bp 201 amino acids = 23.6 kDa Product: baculovirus ORF603 protein |
ORF1629 529 .. 1117 = 589 bp 195 amino acids = 22.3 kDa Product: baculovirus capsid-associated protein required for viral replication |
ORF1629 529 .. 1117 = 589 bp 195 amino acids = 22.3 kDa Product: baculovirus capsid-associated protein required for viral replication |
lef2 3030 .. 3154 = 125 bp 40 amino acids = 4.5 kDa Product: baculovirus late expression factor 2 |
lef2 3030 .. 3154 = 125 bp 40 amino acids = 4.5 kDa Product: baculovirus late expression factor 2 |
rabbit globin terminator 231 .. 342 = 112 bp |
rabbit globin terminator 231 .. 342 = 112 bp |
p10 promoter 4839 .. 4948 = 110 bp baculovirus promoter for expression in insect cells |
p10 promoter 4839 .. 4948 = 110 bp baculovirus promoter for expression in insect cells |
ATG 4963 .. 4965 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 4963 .. 4965 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
Strep-Tag II 4972 .. 4995 = 24 bp 8 amino acids = 1.1 kDa Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin |
Strep-Tag II 4972 .. 4995 = 24 bp 8 amino acids = 1.1 kDa Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin |