pTriEx-5 Ek_LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-enterokinase and a C-terminal 10xHis.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidsInsect Cell VectorspTriEx-5 Ek_LIC (linearized)
Bsu36I (67) NcoI (4961) BstBI (4988) End (5004) Start (0) DraIII (57) pTriEx™-5 Ek/LIC 5004 bp
Bsu36I  (67)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4961)
1 site		 C C A T G G G G T A C C
BstBI  (4988)
1 site		 T T C G A A A A G C T T
End  (5004)
0 sites
Start  (0)
0 sites
DraIII  (57)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1927 .. 2718  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2719 .. 2787  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629