pTriEx-5 Ek_LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-enterokinase and a C-terminal 10xHis.

Sequence Author: MilliporeSigma (Novagen)

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No matches
Bsu36I (67) NcoI (4961) BstBI (4988) End (5004) Start (0) DraIII (57) pTriEx™-5 Ek/LIC 5004 bp
Bsu36I  (67)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4961)
1 site
C C A T G G G G T A C C
BstBI  (4988)
1 site
T T C G A A A A G C T T
End  (5004)
0 sites
Start  (0)
0 sites
DraIII  (57)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1927 .. 2718  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2719 .. 2787  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1927 .. 2787  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3030 .. 3857  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
412 .. 1117  =  706 bp
contains part of ORF1629
ori
1327 .. 1914  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1327 .. 1914  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
Exon 1
4446 .. 4547  =  102 bp
Exon 1
4446 .. 4547  =  102 bp
intron
4548 .. 4711  =  164 bp
intron
4548 .. 4711  =  164 bp
Exon 2
4712 .. 5016  =  305 bp
Exon 2
4712 .. 5016  =  305 bp
CMV enhancer
3925 .. 4228  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3925 .. 4228  =  304 bp
human cytomegalovirus immediate early enhancer
Exon 2
1 .. 233  =  233 bp
Exon 2
1 .. 233  =  233 bp
CMV promoter
4230 .. 4429  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4230 .. 4429  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
T7 terminator
352 .. 399  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
352 .. 399  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
3192 .. 3797  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3192 .. 3797  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
529 .. 1117  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
529 .. 1117  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3030 .. 3154  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3030 .. 3154  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
231 .. 342  =  112 bp
rabbit globin terminator
231 .. 342  =  112 bp
p10 promoter
4839 .. 4948  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
4839 .. 4948  =  110 bp
baculovirus promoter for expression in insect cells
ATG
4963 .. 4965  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4963 .. 4965  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
4972 .. 4995  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
Strep-Tag II
4972 .. 4995  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
enterokinase site
5002 .. 5016  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
5002 .. 5016  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
25 .. 54  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lac operator
4799 .. 4823  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4799 .. 4823  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be r