pBAC-5

Baculovirus transfer plasmid with the gp64 promoter, encoding an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
DraIII (5225) ScaI (4553) NmeAIII (4221) BpmI (4143) BmrI (4113) AlwNI (3596) PspFI (3488) BseYI (3484) PciI (3180) PmeI (3060) SgrAI (2772) BsmI (85) BstXI (315) NsiI (332) MluI (547) BbvCI - Bpu10I (564) BclI * (1156) BglII (1161) SpeI (1323) NcoI (1370) ATG 6xHis SacII (1400) thrombin site PflMI (1461) NheI (1471) BmtI (1475) enterokinase site BseRI - TspMI - XmaI (1503) SmaI - SrfI (1505) StuI * (1523) BamHI (1527) EcoRI (1533) Eco53kI (1541) SacI (1543) HindIII (1546) EagI - NotI (1553) PaeR7I - PspXI - XhoI (1561) 6xHis AvrII (1588) BlpI (1597) SphI (1607) SnaBI (1684) SwaI (1774) ZraI (1963) AatII (1965) BmgBI (2188) AgeI (2532) AleI (2763) pBAC™-5 5517 bp
DraIII  (5225)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (4553)
1 site
A G T A C T T C A T G A
NmeAIII  (4221)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (4143)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmrI  (4113)
1 site
A C T