pBACgus-2cp

Baculovirus transfer plasmid with the polyhedrin promoter, encoding β-glucuronidase plus an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
DraIII (7268) ScaI (6596) NmeAIII (6264) BpmI (6186) PspFI (5531) BseYI (5527) PmeI (5103) SgrAI (4815) AgeI (4575) BmgBI (4231) AatII (4008) ZraI (4006) SwaI (3817) SphI (3650) BlpI (3640) BstXI (315) NsiI (332) BbvCI - Bpu10I (564) baculovirus recombination region (lef2/ORF603) BstBI (1882) MscI (2356) PshAI (2404) BglII (3310) NcoI (3413) ATG 6xHis SacII (3443) thrombin site PflMI (3504) NheI (3514) BmtI (3518) enterokinase site BseRI - TspMI - XmaI (3546) SmaI - SrfI (3548) StuI * (3566) BamHI (3570) EcoRI (3576) Eco53kI (3584) SacI (3586) HindIII (3589) EagI - NotI (3596) PaeR7I - PspXI - XhoI (3604) 6xHis AvrII (3631) pBACgus-2cp 7560 bp
DraIII  (7268)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (6596)
1 site
A G T A C T T C A T G A
NmeAIII  (6264)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (6186)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PspFI  (5531)
1 site
C C C A G C G G G T C G
BseYI  (5527)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PmeI  (5103)
1 site
G T T T A A A C C A A A T T T G
SgrAI  (4815)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AgeI  (4575)
1 site
A C C G G T T G G C C A
BmgBI  (4231)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
AatII  (4008)
1 site
G A C G T C C T G C A G
ZraI  (4006)
1 site
G A C G T C C T G C A G
SwaI  (3817)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
SphI  (3650)
1 site
G C A T G C C G T A C G
BlpI  (3640)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BstXI  (315)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NsiI  (332)
1 site
A T G C A T T A C G T A
BbvCI  (564)
1 site
C C T C A G C G G A G T C G
Bpu10I  (564)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstBI  (1882)
1 site
T T C G