pBAC4x-1

Baculovirus transfer plasmid with two polyhedrin and two p10 promoters, for the simultaneous expression of up to four proteins.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidsInsect Cell VectorspBAC4x-1
DraIII (5625) ScaI (4953) NmeAIII (4621) BpmI (4543) BmrI (4513) AlwNI (3996) PspFI (3888) BseYI (3884) PciI (3580) PmeI (3460) SgrAI (3172) AleI (3163) BsmI (85) BstXI (315) BbvCI - Bpu10I (564) poly(A) signal Bsu36I (1223) MCS EcoRI (1237) BglII (1243) PpuMI (1357) StuI (1495) MCS XbaI (1498) polyhedrin promoter PspOMI (1621) ApaI (1625) TspMI - XmaI (1744) SmaI (1746) MCS SpeI (1750) poly(A) signal polyhedrin promoter BamHI (1937) HindIII (1946) MCS EagI - NotI (1953) PaeR7I - PspXI - XhoI (1961) AvrII - StyI (1988) BlpI (1997) SphI (2007) SnaBI (2084) SwaI (2174) ZraI (2363) AatII (2365) BmgBI (2588) AgeI (2932) pBAC4x-1 5917 bp
DraIII  (5625)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
ScaI  (4953)
1 site		 A G T A C T T C A T G A
NmeAIII  (4621)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (4543)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmrI  (4513)
1 site		 A C T G G G ( N ) 4 N T