pTriEx-3

Insect, bacterial, and mammalian vector with a CMV promoter for expressing proteins with a C-terminal HSV-8xHis cassette.

Sequence Author: MilliporeSigma (Novagen)

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AanI (3391) AanI (2834) AanI (320) AanI (409) CMV enhancer NcoI (2112) EcoRV (2121) TspMI - XmaI (2124) SmaI (2126) Eco53kI (2131) SacI (2133) BamHI (2137) EcoRI (2143) BglII (2151) AscI - BssHII (2159) PstI - SbfI (2169) Acc65I (2177) AgeI (2180) KpnI (2181) BstBI (2186) HindIII (2189) EagI - NotI (2196) PvuII (2206) BstZ17I (2211) PmlI (2216) PaeR7I - XhoI (2258) DraIII (2290) Bsu36I (2300) pTriEx™-3 5082 bp
AanI  (3391)
4 sites
T T A T A A A A T A T T
AanI  (2834)
4 sites
T T A T A A A A T A T T
AanI  (320)
4 sites
T T A T A A A A T A T T
AanI  (409)
4 sites
T T A T A A A A T A T T
NcoI  (2112)
1 site
C C A T G G G G T A C C
EcoRV  (2121)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (2124)
1 site
C C C G G G G G G C C C
XmaI  (2124)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2126)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Eco53kI  (2131)
1 site
G A G C T C C T C G A G
SacI  (2133)
1 site
G A G C T C C T C G A G
BamHI  (2137)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2143)
1 site
G A A T T C C T T A A G
BglII  (2151)
1 site
A G A T C T T C T A G A
AscI  (2159)
1 site
G G C G C G C C C C G C G C G G
BssHII  (2159)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PstI  (2169)
1 site
C T G C A G G A C G T C
SbfI  (2169)
1 site
C C T G C A G G G G A C G T C C
Acc65I  (2177)
1 site
G G T A C C C C A T G G
AgeI  (2180)
1 site
A C C G G T T G G C C A
KpnI  (2181)
1 site
G G T A C C C C A T G G
BstBI  (2186)
1 site
T T C G A A A A G C T T
HindIII  (2189)
1 site
A A G C T T T T C G A A
EagI  (2196)
1 site
C G G