Insect, bacterial, and mammalian vector with a CMV promoter for expressing proteins with a C-terminal HSV-8xHis cassette.
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI can be used at 37°C for brief incubations.
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BssHII is typically used at 50°C, but is 75% active at 37°C.