pIEx Bac-5 3C_LIC (linearized)

Linearized insect cell and baculovirus vector for ligation-independent cloning (LIC), encoding a cleavable N-terminal GST tag and a cleavable C-terminal Strep-Tag® II.

Sequence Author: MilliporeSigma (Novagen)

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DraIII (4024) BanII (3950) NmeAIII (3020) BpmI (2942) AlwNI (2395) PspFI (2287) BseYI (2283) PmeI (1859) SgrAI (1571) AleI (1562) AgeI (1331) BmgBI (987) AatII (764) ZraI (762) SnaBI (483) BstXI (4631) NsiI (4648) BbvCI - Bpu10I (4880) BglII (5477) EcoRV (5750) NheI (6117) BmtI (6121) PvuII (6287) BsiWI (6295) EagI (6353) PfoI (6594) PpuMI (6598) BspEI (6711) BamHI (6745) BtgI - NcoI (6749) EcoNI (6761) BspQI - SapI (6830) MscI (6958) End (7449) Start (0) thrombin site AvrII (72) StuI (77) Bsu36I (79) IE1 terminator PflFI - Tth111I (332) BlpI (396)