pTriEx-4 Ek_LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal 6xHis-S-Tag and a C-terminal HSV-8xHis.

Sequence Author: MilliporeSigma (Novagen)

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AanI (1287) AanI (730) Bsu36I (196) AanI (3298) AanI (3387) CMV enhancer NcoI (5090) XcmI (5116) PflMI (5160) TspMI - XmaI (5192) SmaI (5194) End (5223) Start (0) EcoRV (20) Eco53kI (27) SacI (29) BamHI (33) EcoRI (39) BglII (47) AscI - BssHII (55) PstI - SbfI (65) Acc65I (73) AgeI (76) KpnI (77) BstBI (82) HindIII (85) EagI - NotI (92) PvuII (102) BstZ17I (107) PmlI (112) PaeR7I - XhoI (154) DraIII (186) pTriEx™-4 Ek/LIC 5223 bp