pTriEx-1.1

Insect, bacterial, and mammalian vector for expressing proteins with a C-terminal HSV-8xHis cassette.

Sequence Author: MilliporeSigma (Novagen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
AanI (3610) AanI (3053) AanI (320) AanI (409) CMV enhancer NcoI (2331) EcoRV (2340) TspMI - XmaI (2343) SmaI (2345) Eco53kI (2350) SacI (2352) BamHI (2356) EcoRI (2362) BglII (2370) BfuAI - BspMI (2377) AscI (2378) PstI - SbfI (2388) Acc65I (2396) AgeI (2399) KpnI (2400) BstBI (2405) HindIII (2408) NotI (2415) PvuII (2425) BstZ17I (2430) PmlI (2435) PaeR7I - XhoI (2477) DraIII (2509) pTriEx™-1.1 5301 bp
AanI  (3610)
4 sites
T T A T A A A A T A T T
AanI  (3053)
4 sites
T T A T A A A A T A T T
AanI  (320)
4 sites
T T A T A A A A T A T T
AanI  (409)
4 sites
T T A T A A A A T A T T
NcoI  (2331)
1 site
C C A T G G G G T A C C
EcoRV  (2340)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (2343)
1 site
C C C G G G G G G C C C
XmaI  (2343)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2345)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Eco53kI  (2350)
1 site
G A G C T C C T C G A G
SacI  (2352)
1 site
G A G C T C C T C G A G
BamHI  (2356)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2362)
1 site
G A A T T C C T T A A G
BglII  (2370)
1 site
A G A T C T T C T A G A
BfuAI  (2377)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2377)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AscI  (2378)
1 site
G G C G C G C C C C G C G C G G
PstI  (2388)
1 site
C T G C A G G A C G T C
SbfI  (2388)
1 site
C C T G C A G G G G A C G T C C
Acc65I  (2396)
1 site
G G T A C C C C A T G G
AgeI  (2399)
1 site
A C C G G T T G G C C A
KpnI  (2400)
1 site
G G T A C C C C A T G G
BstBI  (2405)
1 site
T T C G A A A A G C T T
HindIII  (2408)
1 site
A A G C T T T T C G A A
NotI  (2415)
1 site
G C G G C C G C C G C C G G C G
PvuII  (2425)
1 site
C A G C T G G T C G A C
BstZ17I  (2430)
1 site
G T A T A C C A T A T G
PmlI  (2435)
1 site
C A C G T G G T G C A C
PaeR7I  (2477)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2477)
1 site
C T C G A G G A G C T C
DraIII  (2509)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Exon 1
1727 .. 1819  =  93 bp
Exon 1
1727 .. 1819  =  93 bp
Intron
1820 .. 2081  =  262 bp
Intron
1820 .. 2081  =  262 bp
Exon 2
2082 .. 2685  =  604 bp
Exon 2
2082 .. 2685  =  604 bp
AmpR
4379 .. 5239  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4379 .. 5170  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4379 .. 5239  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5171 .. 5239  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4379 .. 5239  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
2864 .. 3569  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
2864 .. 3569  =  706 bp
contains part of ORF1629
ori
3779 .. 4366  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3779 .. 4366  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
1140 .. 1443  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1140 .. 1443  =  304 bp
human cytomegalovirus immediate early enhancer
chicken β-actin promoter
1449 .. 1726  =  278 bp
chicken β-actin promoter
1449 .. 1726  =  278 bp
T7 terminator
2804 .. 2851  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
2804 .. 2851  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
2981 .. 3569  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
2981 .. 3569  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ATG
2333 .. 2335  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2333 .. 2335  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
HSV tag
2444 .. 2476  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
HSV tag
2444 .. 2476  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
8xHis
2483 .. 2506  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
2483 .. 2506  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
2683 .. 2794  =  112 bp
rabbit globin terminator
2683 .. 2794  =  112 bp
p10 promoter
2209 .. 2318  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
2209 .. 2318  =  110 bp
baculovirus promoter for expression in insect cells
lac operator
2169 .. 2193  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2169 .. 2193  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
2150 .. 2168  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2150 .. 2168  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
2320 .. 2325  =  6 bp
ribosome binding site
RBS
2320 .. 2325  =  6 bp
ribosome binding site
poly(A) signal
2661 .. 2666  =  6 bp
poly(A) signal
2661 .. 2666  =  6 bp
ORF:  1315 .. 1704  =  390 bp
ORF:  129 amino acids  =  13.9 kDa
ORF:  1547 .. 1798  =  252 bp
ORF:  83 amino acids  =  8.7 kDa
ORF:  4509 .. 4775  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5289 .. 305  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  343 .. 948  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  387 .. 617  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  1671 .. 2060  =  390 bp
ORF:  129 amino acids  =  13.3 kDa
ORF:  2300 .. 2647  =  348 bp
ORF:  115 amino acids  =  13.0 kDa
ORF:  2981 .. 3598  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  4379 .. 5239  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pTriEx-1.1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.