M13mp18

M13 bacteriophage vector containing a multiple cloning site (MCS). The MCS is reversed relative to M13mp19.

Sequence Author: New England Biolabs

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BglII (6934) SwaI (6785) AleI (6576) Bsu36I (6508) BglI (6436) FspI (6426) PvuI (6407) BmrI (6326) HindIII (6281) SphI (6279) PstI - SbfI (6273) HincII (6265) AccI (6264) SalI (6263) XbaI (6257) BamHI (6251) SmaI (6248) KpnI - TspMI - XmaI (6246) Acc65I (6242) SacI (6240) Eco53kI (6238) EcoRI (6230) lac operator BtsI - BtsαI (6094) PluTI (6004) SfoI (6002) BsaHI - NarI * (6001) KasI (6000) BsmBI - Esp3I (5976) DrdI (5764) DraIII (5720) NaeI (5614) BsrFI - NgoMIV (5612) MscI (5081) PacI (4135) BsrGI (1021) M13 gene VII SnaBI (1270) BspHI (1299) BsmI (1751) BseRI (2022) BaeGI - Bme1580I (2091) AlwNI (2191) AfeI (3040) M13mp18 7249 bp
BglII  (6934)
1 site
A G A T C T T C T A G A
SwaI  (6785)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AleI  (6576)
1 site
C A C N N N N G T G G T G N N N N C A C
Bsu36I  (6508)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BglI  (6436)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (6426)
1 site
T G C G C A A C G C G T
PvuI  (6407)
1 site
C G A T C G G C T A G C
BmrI  (6326)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
HindIII  (6281)
1 site
A A G C T T T T C G A A
SphI  (6279)
1 site
G C A T G C C G T A C G
PstI  (6273)
1 site
C T G C A G G A C G T C
SbfI  (6273)
1 site
C C T G C A G G G G A C G T C C
HincII  (6265)
1 site
G T Y R A C C A R Y T G
AccI  (6264)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (6263)
1 site
G T C G A C C A G C T G
XbaI  (6257)
1 site
T C T A G A A G A T C T
BamHI  (6251)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (6248)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (6246)
1 site
G G T A C C C C A T G G
TspMI  (6246)
1 site
C C C G G G G G G C C C
XmaI  (6246)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Acc65I  (6242)
1 site
G G T A C C C C A T G G
SacI  (6240)
1 site
G A G C T C C T C G A G
Eco53kI  (6238)
1 site
G A G C T C C T C G A G
EcoRI  (6230)
1 site
G A A T T C C T T A A G
BtsI  (6094)
1 site
G C A G T G N N C G T C A C
BtsαI  (6094)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
PluTI  (6004)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (6002)
1 site
G G C G C C C C G C G G
BsaHI  (6001)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (6001)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (6000)
1 site
G G C G C C C C G C G G
BsmBI  (5976)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (5976)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
DrdI  (5764)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
DraIII  (5720)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (5614)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsrFI  (5612)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NgoMIV  (5612)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
MscI  (5081)
1 site
T G G C C A A C C G G T
PacI  (4135)
1 site
T T A A T T A A A A T T A A T T
BsrGI  (1021)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SnaBI  (1270)
1 site
T A C G T A A T G C A T
BspHI  (1299)
1 site
T C A T G A A G T A C T
BsmI  (1751)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BseRI  (2022)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BaeGI  (2091)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (2091)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
AlwNI  (2191)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from diff