pOSIP-KT

Prokaryotic one-step cloning and chromosomal integration vector encoding a kanamycin resistance marker and the phage φ21 integrase.
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EcoRI (1) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (6115) BbvCI (5779) BmtI (5643) NheI (5639) StuI (5632) BglI (5405) SnaBI (4648) SalI (4563) PpuMI * (4356) PvuI (4107) EcoNI (4019) NruI (3764) DraIII (3576) Eco53kI (9) SacI (11) Acc65I (13) KpnI (17) BamHI (22) BssHII (70) BsrGI (198) SrfI (225) BglII (469) SpeI (1126) PstI (1144) PaeR7I - XhoI (1152) KasI (1164) NarI (1165) SfoI (1166) PluTI (1168) MfeI (1239) PacI (1340) PspOMI (1349) ApaI (1353) AvrII (1418) EarI (1442) BstAPI (1965) PshAI (2090) MluI (2240) BtsI - BtsαI (2887) CsiI - SexAI * (3200) pOSIP-KT 6944 bp
EcoRI  (1)
1 site
G A A T T C C T T A A G
PflFI  (6115)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (6115)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BbvCI  (5779)
1 site
C C T C A G C G G A G T C G
BmtI  (5643)
1 site
G C T A G C C G A T C G
NheI  (5639)
1 site
G C T A G C C G A T C G
StuI  (5632)
1 site
A G G C C T T C C G G A
BglI  (5405)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SnaBI  (4648)
1 site
T A C G T A A T G C A T
SalI  (4563)
1 site
G T C G A C C A G C T G
PpuMI  (4356)
1 site
R G G W C C Y Y C C W G G R
* Blocked by Dcm methylation.
Sticky ends from different PpuMI sites may not be compatible.
PvuI  (4107)
1 site
C G A T C G G C T A G C
EcoNI  (4019)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
NruI  (3764)
1 site
T C G C G A A G C G C T
DraIII  (3576)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
Eco53kI  (9)
1 site
G A G C T C C T C G A G
SacI  (11)
1 site
G A G C T C C T C G A G
Acc65I  (13)
1 site
G G T A C C C C A T G G
KpnI  (17)
1 site
G G T A C C C C A T G G
BamHI  (22)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BssHII  (70)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BsrGI  (198)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SrfI  (225)
1 site
G C C C G G G C C G G G C C C G
BglII  (469)
1 site
A G A T C T T C T A G A
SpeI  (1126)
1 site
A C T A G T T G A T C A
PstI  (1144)
1 site
C T G C A G G A C G T C
PaeR7I  (1152)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1152)
1 site
C T C G A G G A G C T C
KasI  (1164)
1 site
G G C G C C C C G C G G
NarI  (1165)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1166)
1 site
G G C G C C C C G C G G
PluTI  (1168)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
MfeI  (1239)
1 site
C A A T T G G T T A A C
PacI  (1340)
1 site
T T A A T T A A A A T T A A T T
PspOMI  (1349)
1 site
G G G C C C C C C G G G
ApaI  (1353)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (1418)
1 site
C C T A G G G G A T C C
EarI  (1442)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BstAPI  (1965)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PshAI  (2090)
1 site
G A C N N N N G T C C T G N N N N C A G