pOSIP-CH
Prokaryotic one-step cloning and chromosomal integration vector encoding a chloramphenicol resistance marker and the HK022 integrase.
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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