pOSIP-CH

Prokaryotic one-step cloning and chromosomal integration vector encoding a chloramphenicol resistance marker and the HK022 integrase.
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HomePlasmidsBasic Cloning VectorspOSIP-CH
Acc65I (13) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (5569) λ tL3 terminator BseRI (5221) BmtI (5097) NheI (5093) NdeI (4824) SalI (4326) ScaI (4175) SspI (4070) PasI (3990) BpmI (3880) BspEI (3754) PvuII (3658) DraIII (3415) KpnI (17) BamHI (22) BssHII (70) BsrGI (198) SrfI (225) BsaI (363) BglII (469) BciVI (923) SpeI (1126) PstI (1144) SphI (1150) PaeR7I - XhoI (1152) KasI (1164) NarI (1165) SfoI (1166) PluTI (1168) BstBI (1234) MfeI (1239) PacI (1340) AgeI (1343) PspOMI (1349) ApaI (1353) ZraI (1379) AatII (1381) AvrII (1418) BstAPI (1965) PshAI (2090) AflIII - MluI (2240) KflI - PpuMI (2350) TaqII (2526) NruI (2828) BtsI - BtsαI (3076) pOSIP-CH 6398 bp
Acc65I  (13)
1 site		 G G T A C C C C A T G G
PflFI  (5569)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5569)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BseRI  (5221)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BmtI  (5097)
1 site		 G C T A G C C G A T C G
NheI  (5093)
1 site		 G C T A G C C G A T C G
NdeI  (4824)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SalI  (4326)
1 site		 G T C G A C C A G C T G
ScaI  (4175)
1 site		 A G T A C T T C