pOSIP-TT

Prokaryotic one-step cloning and chromosomal integration vector encoding a tetracycline resistance marker and the phage φ21 integrase.
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HomePlasmidsBasic Cloning VectorspOSIP-TT
EcoRI (1) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (6566) λ tL3 terminator BmtI (6094) NheI (6090) SnaBI (5099) SalI (5014) ScaI (4481) PmlI (4204) BspDI - ClaI (3582) Acc65I (13) KpnI (17) BamHI (22) BssHII (70) SrfI (225) BglII (469) SpeI (1126) PstI (1144) PaeR7I - XhoI (1152) PacI (1340) PspOMI (1349) ApaI (1353) AvrII (1418) EarI (1442) PshAI (2090) BtsI - BtsαI (2887) CsiI - SexAI * (3200) DraIII (3576) pOSIP-TT 7395 bp
EcoRI  (1)
1 site		 G A A T T C C T T A A G
PflFI  (6566)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (6566)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmtI  (6094)
1 site		 G C T A G C C G A T C G
NheI  (6090)
1 site		 G C T A G C C G A T C G
SnaBI  (5099)
1 site		 T A C G T A A T G C A T
SalI  (5014)
1 site		 G T C G A C C A G C T G
ScaI  (4481)
1 site		 A G T A C T T C A T G A
PmlI  (4204)
1 site		 C A C G T G G T G C A C
BspDI  (3582)
1 site		 A T C G A T T A G C T A
ClaI  (3582)
1 site		 A T C G A T T A G C T A
Acc65I  (13)
1 site		 G G T A C C C C A T G G
KpnI  (17)
1 site		 G G T A C C C C A T G G
BamHI  (22)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BssHII  (70)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
SrfI  (225)
1 site		 G C C C G G G C C G G G C C C G
BglII  (469)
1 site		 A G A T C T T C T A G A
SpeI  (1126)
1 site		 A C T A G T T G A T C A
PstI  (1144)
1 site		 C T G C A G G A C G T C
PaeR7I  (1152)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1152)
1 site		 C T C G A G G A G C T C
PacI  (1340)
1 site		 T T A A T T A A A A T T A A T