pOSIP-TT

Prokaryotic one-step cloning and chromosomal integration vector encoding a tetracycline resistance marker and the phage φ21 integrase.
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HomePlasmidsBasic Cloning VectorspOSIP-TT
EcoRI (1) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (6566) λ tL3 terminator BmtI (6094) NheI (6090) SnaBI (5099) SalI (5014) ScaI (4481) PmlI (4204) BspDI - ClaI (3582) Acc65I (13) KpnI (17) BamHI (22) BssHII (70) SrfI (225) BglII (469) SpeI (1126) PstI (1144) PaeR7I - XhoI (1152) PacI (1340) PspOMI (1349) ApaI (1353) AvrII (1418) EarI (1442) PshAI (2090) BtsI - BtsαI (2887) CsiI - SexAI * (3200) DraIII (3576) pOSIP-TT 7395 bp
EcoRI  (1)
1 site		 G A A T T C C T T A A G
PflFI  (6566)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (6566)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmtI  (6094)
1 site		 G C T A G C C G A T C G
NheI  (6090)
1 site		 G C T A G C C G A T C G
SnaBI  (5099)
1 site		 T A C G T A A T G C A T
SalI  (5014)
1 site		 G T C G A C C A G C T G
ScaI  (4481)
1 site		 A G T A C T T C A T G