pBeloBAC11

Single-copy E. coli plasmid vector designed for constructing Bacterial Artificial Chromosomes (BACs).

Sequence Author: New England Biolabs

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HomePlasmidsBasic Cloning VectorspBeloBAC11
HpaI (6997) BstEII (6972) CsiI - SexAI * (6968) AhdI (6854) PciI (6850) ApaI (6340) PspOMI (6336) BsaI (6178) PfoI * (6119) SpeI (6090) AleI (5911) BclI * (5166) BseRI (5068) SnaBI (4999) PflFI - Tth111I (4639) BstXI (4453) MfeI (4355) NmeAIII (4307) AfeI (3934) ApaLI (87) KasI (145) BsaHI - NarI (146) SfoI (147) PluTI (149) BamHI (354) SphI (382) HindIII (384) lac operator SfiI - SrfI (640) ScaI (794) BstZ17I (1833) PaeR7I - PspXI - XhoI (2381) PsiI (2490) StuI (2542) EcoNI (2837) FspAI (3120) BsrGI (3148) BlpI (3847) pBeloBAC11 7507 bp
HpaI  (6997)
1 site		 G T T A A C C A A T T G
BstEII  (6972)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
CsiI  (6968)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (6968)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AhdI  (6854)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (6850)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
ApaI  (6340)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (6336)
1 site		 G G G C C C C C C G G G
BsaI  (6178)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (6119)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SpeI  (6090)
1 site		 A C T A G T T G A T C A
AleI  (5911)
1 site		 C A C N N N N G T G G T G N N N N C A C
BclI  (5166)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BseRI  (5068)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SnaBI  (4999)
1 site		 T A C G T A A T G C A T
PflFI  (4639)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4639)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstXI  (4453)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
MfeI  (4355)
1 site		 C A A T T G G T T A A C
NmeAIII  (4307)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AfeI  (3934)
1 site		 A G C G C T T C G C G A
ApaLI  (87)
1 site		 G T G C A C C A C G T G
KasI  (145)
1 site		 G G C G C C C C G C G G
BsaHI  (146)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (146)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (147)
1 site		 G G C G C C C C G C G G
PluTI  (149)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BamHI  (354)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SphI  (382)
1 site		 G C A T G C C G T A C G
HindIII  (384)
1 site		 A A G C T T T T C G A A
SfiI  (640)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SrfI  (640)
1 site		 G C C C G G G C C G G G C C C G
ScaI  (794)
1 site		 A G T A C T T C A T G A
BstZ17I  (1833)
1 site		 G T A T A C C A T A T G
PaeR7I  (2381)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2381)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (2381)
1 site		 C T C G A G G A G C T C
PsiI  (2490)
1 site		 T T A T A A A A T A T T
StuI  (2542)
1 site		 A G G C C T T C C G G A
EcoNI  (2837)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
FspAI  (3120)
1 site		 R T G C G C A Y Y A C G C G T R
BsrGI  (3148)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BlpI  (3847)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
sopA
4099 .. 5274  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
4099 .. 5274  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
repE
2765 .. 3520  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
2765 .. 3520  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
CmR
766 .. 1425  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
766 .. 1425  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
sopC
6318 .. 6791  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
6318 .. 6791  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
cos
7051 .. 7449  =  399 bp
lambda cos site; allows packaging into phage lambda particles
cos
7051 .. 7449  =  399 bp
lambda cos site; allows packaging into phage lambda particles
lacZα
56 .. 436  =  381 bp
126 amino acids  =  14.5 kDa
Product: LacZα fragment of β-galactosidase
lacZα
56 .. 436  =  381 bp
126 amino acids  =  14.5 kDa
Product: LacZα fragment of β-galactosidase
incC
3523 .. 3773  =  251 bp
incompatibility region of the bacterial F plasmid
incC
3523 .. 3773  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
2455 .. 2674  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
2455 .. 2674  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
cat promoter
1426 .. 1528  =  103 bp
promoter of the E. coli cat gene
cat promoter
1426 .. 1528  =  103 bp
promoter of the E. coli cat gene
loxP
7467 .. 7500  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
7467 .. 7500  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
lac promoter
480 .. 510  =  31 bp
3 segments
   Segment 3:  -10  
   480 .. 486  =  7 bp
promoter for the E. coli lac operon
lac promoter
480 .. 510  =  31 bp
3 segments
   Segment 2:  
   487 .. 504  =  18 bp
promoter for the E. coli lac operon
lac promoter
480 .. 510  =  31 bp
3 segments
   Segment 1:  -35  
   505 .. 510  =  6 bp
promoter for the E. coli lac operon
lac promoter
480 .. 510  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
456 .. 472  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
456 .. 472  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
sopB
5274 .. 6245  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
5274 .. 6245  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
MCS
333 .. 389  =  57 bp
pUC18/19 multiple cloning site
MCS
333 .. 389  =  57 bp
pUC18/19 multiple cloning site
T7 promoter
312 .. 330  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
312 .. 330  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
396 .. 414  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
396 .. 414  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
M13 fwd
289 .. 305  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
289 .. 305  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
432 .. 448  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
432 .. 448  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  4099 .. 5274  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
ORF:  5626 .. 6024  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  2765 .. 3520  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
ORF:  525 .. 812  =  288 bp
ORF:  95 amino acids  =  10.6 kDa
ORF:  1644 .. 1991  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  2754 .. 2984  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  3069 .. 3314  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  5274 .. 6245  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  56 .. 436  =  381 bp
ORF:  126 amino acids  =  14.5 kDa
ORF:  2921 .. 3154  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  5315 .. 5551  =  237 bp
ORF:  78 amino acids  =  8.3 kDa
ORF:  766 .. 1425  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  1617 .. 1868  =  252 bp
ORF:  83 amino acids  =  9.6 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)