pOSIP-CT

Prokaryotic one-step cloning and chromosomal integration vector encoding a chloramphenicol resistance marker and the phage φ21 integrase.
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Acc65I (13) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (6039) λ tL3 terminator BbvCI (5703) BmtI (5567) NheI (5563) StuI (5556) BglI (5329) SnaBI (4572) SalI (4487) ScaI (4336) PasI (4151) BpmI (4041) BspEI (3915) PvuII (3819) DraIII (3576) KpnI (17) BamHI (22) BssHII (70) BsrGI (198) SrfI (225) BglII (469) SpeI (1126) PstI (1144) PaeR7I - XhoI (1152) KasI (1164) NarI (1165) SfoI (1166) PluTI (1168) MfeI (1239) PacI (1340) PspOMI (1349) ApaI (1353) AvrII (1418) EarI (1442) BstAPI (1965) PshAI (2090) MluI (2240) BtsI - BtsαI (2887) CsiI - SexAI * (3200) pOSIP-CT 6868 bp
Acc65I  (13)
1 site
G G T A C C C C A T G G
PflFI  (6039)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (6039)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BbvCI  (5703)
1 site
C C T C A G C G G A G T C G
BmtI  (5567)
1 site
G C T A G C C G A T C G
NheI  (5563)
1 site
G C T A G C C G A T C G
StuI  (5556)
1 site
A G G C C T T C C G G A
BglI  (5329)
1 site
G C C N N N N N G G C C G G N N N N