pLATE11 (linearized)
Vector for ligation independent cloning (LIC) and tightly regulated bacterial expression of an untagged protein.
Sequence Author: Thermo Fisher
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
|
|
|
|
|
|
| ||
Sticky ends from different BglI sites may not be compatible. |
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
Sticky ends from different AlwNI sites may not be compatible. |
| ||
PciI is inhibited by nonionic detergents. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
|
| ||
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
|
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
| ||
Sticky ends from different PfoI sites may not be compatible. |
|
|
|
|
|
|
| ||
Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
| ||
Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
|
|
|
| ||
SwaI is typically used at 25°C, but is 50% active at 37°C. |
|
|
|
|
|
|
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
| ||
Sticky ends from different StyI sites may not be compatible. |
| ||
Sticky ends from different PflMI sites may not be compatible. |
|
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
|
| ||
ApaI can be used between 25°C and 37°C. |
| ||
ApoI is typically used at 50°C, but is 50% active at 37°C. |
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
|
| ||
| * Blocked by Dcm methylation. Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
| ||
Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
|