pLATE11 (linearized)

Vector for ligation independent cloning (LIC) and tightly regulated bacterial expression of an untagged protein.

Sequence Author: Thermo Fisher

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
rrnB T1 terminator AatII (4004) ZraI (4002) SspI (3886) ScaI (3562) PvuI (3452) FspI (3304) BglI (3202) AhdI (3082) AlwNI (2605) PciI (2189) BspQI - SapI (2073) NdeI (2012) BstZ17I (1962) AccI (1961) BsaAI (1943) PflFI - Tth111I (1936) PfoI (1833) AfeI (1445) rrnB T2 terminator LIC reverse sequencing primer (4297 .. 4312) lac operator Eco53kI (4315) SacI (4317) Acc65I (4319) KpnI (4323) MauBI (4330) BfuAI - BspMI - PaqCI (4335) SbfI (4346) AscI (4351) LIC forward sequencing primer (4360 .. 4379) T7 promoter LIC reverse sequencing primer (4387 .. 4401) XbaI (4407) SwaI (4432) End (4438) Start (0) PmeI (18) StuI (25) SphI (33) PacI (55) LIC reverse sequencing primer (62 .. 85) HindIII (123) BspDI - ClaI (130) BamHI (157) StyI (173) T7 terminator PflMI (228) MluI (646) BclI * (660) BstEII (827) PspOMI (853) ApaI (857) ApoI (921) EcoRV (1096) HpaI (1152) KasI (1285) NarI * (1286) SfoI (1287) PluTI (1289) BspEI * (1380) BsaBI * (1388) pLATE11 4438 bp
AatII  (4004)
1 site
G A C G T C C T G C A G
ZraI  (4002)
1 site
G A C G T C C T G C A G
SspI  (3886)
1 site
A A T A T T T T A T A A
ScaI  (3562)
1 site
A G T A C T T C A T G A
PvuI  (3452)
1 site
C G A T C G G C T A G C
FspI  (3304)
1 site
T G C G C A A C G C G T
BglI  (3202)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AhdI  (3082)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2605)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2189)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2073)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2073)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2012)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (1962)
1 site
G T A T A C C A T A T G
AccI  (1961)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaAI  (1943)
1 site
Y A C G T R R T G C A Y
PflFI  (1936)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1936)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (1833)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
AfeI  (1445)
1 site
A G C G C T T C G C G A
Eco53kI  (4315)
1 site
G A G C T C C T C G A G
SacI  (4317)
1 site
G A G C T C C T C G A G
Acc65I  (4319)
1 site
G G T A C C C C A T G G
KpnI  (4323)
1 site
G G T A C C C C A T G G
MauBI  (4330)
1 site
C G C G C G C G G C G C G C G C
BfuAI  (4335)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4335)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PaqCI  (4335)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SbfI  (4346)
1 site
C C T G C A G G G G A C G T C C
AscI  (4351)
1 site
G G C G C G C C C C G C G C G G
XbaI  (4407)
1 site
T C T A G A A G A T C T
SwaI  (4432)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
End  (4438)
0 sites
Start  (0)
0 sites
PmeI  (18)
1 site
G T T T A A A C C A A A T T T G
StuI  (25)
1 site
A G G C C T T C C G G A
SphI  (33)
1 site
G C A T G C C G T A C G
PacI  (55)
1 site
T T A A T T A A A A T T A A T T
HindIII  (123)
1 site
A A G C T T T T C G A A
BspDI  (130)
1 site
A T C G A T T A G C T A
ClaI  (130)
1 site
A T C G A T T A G C T A
BamHI  (157)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StyI  (173)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
PflMI  (228)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
MluI  (646)
1 site
A C G C G T T G C G C A
BclI  (660)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (827)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (853)
1 site
G G G C C C C C C G G G
ApaI  (857)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
ApoI  (921)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRV  (1096)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HpaI  (1152)
1 site
G T T A A C C A A T T G
KasI  (1285)
1 site
G G C G C C C C G C G G
NarI  (1286)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1287)
1 site
G G C G C C C C G C G G
PluTI  (1289)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BspEI  (1380)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
BsaBI  (1388)
1 site
G A T