pMMB190

Broad-host-range vector with an ampicillin resistance gene, suitable for blue-white screening and expression of toxic proteins.
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HomePlasmidsBasic Cloning VectorspMMB190
EcoRI (9064) lac operator lac operator tac promoter MauBI (8688) MluI (8231) ApaI - BanII (8028) PspOMI (8024) HpaI (7729) BsmBI - Esp3I (7616) BspQI - SapI (7423) FspAI (6952) BsaAI (6897) EcoNI (6802) PfoI * (6662) SgrAI (4714) TspMI - XmaI (3) SmaI (5) BamHI (8) SalI (14) PstI (24) HindIII (28) rrnB T2 terminator BssSI - BssSαI (827) DraI (918) AhdI (1496) CsiI - SexAI * (1620) SfiI (1854) EcoO109I (2406) BstZ17I (3229) PasI (4235) pMMB190 9066 bp
EcoRI  (9064)
1 site		 G A A T T C C T T A A G
MauBI  (8688)
1 site		 C G C G C G C G G C G C G C G C
MluI  (8231)
1 site		 A C G C G T T G C G C A
ApaI  (8028)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (8028)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
PspOMI  (8024)
1 site		 G G G C C C C C C G G G
HpaI  (7729)
1 site		 G T T A A C C A A T T G
BsmBI  (7616)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (7616)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
BspQI  (7423)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (7423)
1 site		 G C T C T T C N C G A G A A G N N