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pMMB190
Broad-host-range vector with an ampicillin resistance gene, suitable for blue-white screening and expression of toxic proteins.
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Plasmids
Basic Cloning Vectors
pMMB190
Show Static Map
EcoRI
(9064)
lac operator
lac operator
tac promoter
MauBI
(8688)
MluI
(8231)
ApaI
-
BanII
(8028)
PspOMI
(8024)
HpaI
(7729)
BsmBI
-
Esp3I
(7616)
BspQI
-
SapI
(7423)
FspAI
(6952)
BsaAI
(6897)
EcoNI
(6802)
PfoI
*
(6662)
SgrAI
(4714)
TspMI
-
XmaI
(3)
SmaI
(5)
BamHI
(8)
SalI
(14)
PstI
(24)
HindIII
(28)
rrnB T2 terminator
BssSI
-
BssSαI
(827)
DraI
(918)
AhdI
(1496)
CsiI
-
SexAI
*
(1620)
SfiI
(1854)
EcoO109I
(2406)
BstZ17I
(3229)
PasI
(4235)
XmaI
(3)
XmaI
(3)
XmaI
TspMI
-
(3)
TspMI
-
(3)
TspMI
-
SmaI
(5)
SmaI
(5)
SmaI
BamHI
(8)
BamHI
(8)
BamHI
SalI
(14)
SalI
(14)
SalI
PstI
(24)
PstI
(24)
PstI
HindIII
(28)
HindIII
(28)
HindIII
BssSI
-
(827)
BssSI
-
(827)
BssSI
-
BssSαI
(827)
BssSαI
(827)
BssSαI
DraI
(918)
DraI
(918)
DraI
AhdI
(1496)
AhdI
(1496)
AhdI
CsiI
-
(1620)
CsiI
-
(1620)
CsiI
-
SexAI
*
(1620)
SexAI
*
(1620)
SexAI
*
SfiI
(1854)
SfiI
(1854)
SfiI
EcoO109I
(2406)
EcoO109I
(2406)
EcoO109I
BstZ17I
(3229)
BstZ17I
(3229)
BstZ17I
PasI
(4235)
PasI
(4235)
PasI
SgrAI
(4714)
SgrAI
(4714)
SgrAI
PfoI
*
(6662)
PfoI
*
(6662)
PfoI
*
EcoNI
(6802)
EcoNI
(6802)
EcoNI
BsaAI
(6897)
BsaAI
(6897)
BsaAI
FspAI
(6952)
FspAI
(6952)
FspAI
SapI
(7423)
SapI
(7423)
SapI
BspQI
-
(7423)
BspQI
-
(7423)
BspQI
-
Esp3I
(7616)
Esp3I
(7616)
Esp3I
BsmBI
-
(7616)
BsmBI
-
(7616)
BsmBI
-
HpaI
(7729)
HpaI
(7729)
HpaI
PspOMI
(8024)
PspOMI
(8024)
PspOMI
ApaI
-
(8028)
ApaI
-
(8028)
ApaI
-
BanII
(8028)
BanII
(8028)
BanII
MluI
(8231)
MluI
(8231)
MluI
MauBI
(8688)
MauBI
(8688)
MauBI
EcoRI
(9064)
EcoRI
(9064)
EcoRI
tac promoter
tac promoter
tac promoter
tac promoter
tac promoter
tac promoter
tac promoter
tac promoter
rrnB T2 terminator
rrnB T2 terminator
rrnB T2 terminator
rrnB T2 terminator
lac operator
lac operator
lac operator
lac operator
lac operator
lac operator
lac operator
lac operator
pMMB190
9066 bp
EcoRI (9064)
1 site
G
A
A
T
T
C
C
T
T
A
A
G
MauBI (8688)
1 site
C
G
C
G
C
G
C
G
G
C
G
C
G
C
G
C
MluI (8231)
1 site
A
C
G
C
G
T
T
G
C
G
C
A
ApaI (8028)
1 site
G
G
G
C
C
C
C
C
C
G
G
G
ApaI can be used between 25°C and 37°C.
BanII (8028)
1 site
G
R
G
C
Y
C
C
Y
C
G
R
G
Sticky ends from different BanII sites may not be compatible.
PspOMI (8024)
1 site
G
G
G
C
C
C
C
C
C
G
G
G
HpaI (7729)
1 site
G
T
T
A
A
C
C
A
A
T
T
G
BsmBI (7616)
1 site
C
G
T
C
T
C
N
G
C
A
G
A
G
N
(
N
)
4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I (7616)
1 site