pMMB190
Broad-host-range vector with an ampicillin resistance gene, suitable for blue-white screening and expression of toxic proteins.
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
|
|
|
| ||
ApaI can be used between 25°C and 37°C. |
| ||
Sticky ends from different BanII sites may not be compatible. |
|
|
| ||
Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
| ||
Sticky ends from different Esp3I sites may not be compatible. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
|
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
* Blocked by Dcm methylation. Sticky ends from different PfoI sites may not be compatible. |
| ||
Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
|