pSF-Core
Promoterless backbone SnapFast™ cloning vector, on which all of the other plasmids from Oxford Genetics are based.
Sequence Author: Oxford Genetics
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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The 1-base overhangs produced by BmrI may be hard to ligate.Sticky ends from different BmrI sites may not be compatible.Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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BsaHI is typically used at 37°C, but is even more active at 60°C. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Sticky ends from different SanDI sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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Sticky ends from different BanII sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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