ApaI can be used between 25°C and 37°C.
Sticky ends from different BanII sites may not be compatible.
Sticky ends from different BspQI sites may not be compatible.
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible.
|* Blocked by Dcm methylation.|
Sticky ends from different PfoI sites may not be compatible.
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI can be used at 37°C for brief incubations.
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Sticky ends from different SfcI sites may not be compatible.SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C.