pMMB206
Broad-host-range vector with a chloramphenicol resistance gene, suitable for blue-white screening and expression of toxic proteins.
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different SfcI sites may not be compatible.SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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