pMMB206

Broad-host-range vector with a chloramphenicol resistance gene, suitable for blue-white screening and expression of toxic proteins.
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EcoRI (9311) lac operator lac operator tac promoter MauBI (8935) MluI (8478) ApaI - BanII (8275) PspOMI (8271) HpaI (7976) BspQI - SapI (7670) FspAI (7199) EcoNI (7049) PfoI * (6909) SgrAI (4961) TspMI - XmaI (3) SmaI (5) BamHI (8) SalI (14) SfcI (20) PstI (24) HindIII (28) PvuI (154) XmnI (215) BsaI (363) rrnB T2 terminator BssSI - BssSαI (827) BspEI (1471) SnaBI (1481) SfiI (2101) EcoO109I (2653) BstZ17I (3476) pMMB206 9313 bp
EcoRI  (9311)
1 site
G A A T T C C T T A A G
MauBI  (8935)
1 site
C G C G C G C G G C G C G C G C
MluI  (8478)
1 site
A C G C G T T G C G C A
ApaI  (8275)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (8275)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (8271)
1 site
G G G C C C C C C G G G
HpaI  (7976)
1 site
G T T A A C C A A T T G
BspQI  (7670)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7670)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (7199)
1 site
R T G C G C A Y Y A C G C G T R
EcoNI  (7049)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PfoI  (6909)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SgrAI  (4961)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
TspMI  (3)
1 site
C C C G G G G G G C C C
XmaI  (3)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (5)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (8)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (14)
1 site
G T C G A C C A G C T G
SfcI  (20)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PstI  (24)
1 site
C T G C A G G A C G T C
HindIII  (28)
1 site
A A G C T T T T C G A A
PvuI  (154)
1 site
C G A T C G G C T A G C
XmnI  (215)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (363)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BssSI  (827)
1 site
C A C G A G G T G C T C
BssSαI  (827)
1 site