pMMB206
Broad-host-range vector with a chloramphenicol resistance gene, suitable for blue-white screening and expression of toxic proteins.
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different SfcI sites may not be compatible.SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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| lacI 7751 .. 8833 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| lacI 7751 .. 8833 = 1083 bp 360 amino acids = 38.6 kDa Product: lac repressor The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
| RSF1010 RepB 4315 .. 5286 = 972 bp 323 amino acids = 35.9 kDa Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) |
| RSF1010 RepB 4315 .. 5286 = 972 bp 323 amino acids = 35.9 kDa Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) |
| RSF1010 RepC 6623 .. 7474 = 852 bp 283 amino acids = 31.1 kDa Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) |
| RSF1010 RepC 6623 .. 7474 = 852 bp 283 amino acids = 31.1 kDa Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) |
| CmR 1262 .. 1921 = 660 bp 219 amino acids = 25.6 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 1262 .. 1921 = 660 bp 219 amino acids = 25.6 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| RSF1010 oriV 2253 .. 2647 = 395 bp replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989) |
| RSF1010 oriV 2253 .. 2647 = 395 bp replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989) |
| lacZα 9297 .. 208 = 225 bp 74 amino acids = 8.2 kDa Product: LacZα fragment of β-galactosidase |
| lacZα 9297 .. 208 = 225 bp 74 amino acids = 8.2 kDa Product: LacZα fragment of β-galactosidase |
| cat promoter 1159 .. 1261 = 103 bp promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase |
| cat promoter 1159 .. 1261 = 103 bp promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase |
| RSF1010 oriT 2989 .. 3076 = 88 bp origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) |
| RSF1010 oriT 2989 .. 3076 = 88 bp origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) |
| rrnB T1 terminator 393 .. 479 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
| rrnB T1 terminator 393 .. 479 = 87 bp transcription terminator T1 from the E. coli rrnB gene |
| lacIq promoter 8834 .. 8911 = 78 bp In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold. |
| lacIq promoter 8834 .. 8911 = 78 bp In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold. |
| lac promoter 9223 .. 9253 = 31 bp 3 segments Segment 1: -35 9223 .. 9228 = 6 bp promoter for the E. coli lac operon |
| lac promoter 9223 .. 9253 = 31 bp 3 segments Segment 2: 9229 .. 9246 = 18 bp promoter for the E. coli lac operon |
| lac promoter 9223 .. 9253 = 31 bp 3 segments Segment 3: -10 9247 .. 9253 = 7 bp promoter for the E. coli lac operon |
| lac promoter 9223 .. 9253 = 31 bp 3 segments promoter for the E. coli lac operon |
| tac promoter 9141 .. 9169 = 29 bp 3 segments Segment 1: -35 9141 .. 9146 = 6 bp strong E. coli promoter; hybrid between the trp and lac UV5 promoters |
| tac promoter 9141 .. 9169 = 29 bp 3 segments Segment 2: 9147 .. 9162 = 16 bp strong E. coli promoter; hybrid between the trp and lac UV5 promoters |
| tac promoter 9141 .. 9169 = 29 bp 3 segments Segment 3: -10 9163 .. 9169 = 7 bp strong E. coli promoter; hybrid between the trp and lac UV5 promoters |
| tac promoter 9141 .. 9169 = 29 bp 3 segments strong E. coli promoter; hybrid between the trp and lac UV5 promoters |
| rrnB T2 terminator 571 .. 598 = 28 bp transcription terminator T2 from the E. coli rrnB gene |
| rrnB T2 terminator 571 .. 598 = 28 bp transcription terminator T2 from the E. coli rrnB gene |