pEZ BAC (BamHI linearized)

BamHI-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic inserts.

Sequence Author: Lucigen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
SacII (6773) FseI (6769) BciVI - MreI (6758) XcmI (6568) XbaI (6058) EcoNI (5784) SmaI (5761) TspMI - XmaI (5759) FspAI (5502) BsrGI (5470) SbfI (5233) BlpI (4772) AfeI (4688) MfeI (4263) NdeI (4247) BstXI (4173) KpnI (4047) Acc65I (4043) PflFI - Tth111I (3982) BseRI (3556) BclI* (3452) T3Te terminatorAscI (6994) NotI (7022) BEZ-F1 (7112 .. 7135) BsrBI (7152) lac operatorHindIII (7229) SphI (7239) HpaI (7253) <BamHI> (7260) <BamHI> (0) PmlI (12) EcoRI (17) BEZ-R1 (102 .. 125) KasI (204) NarI (205) SfoI (206) BbeI (208) NotI (234) PmeI (283) EcoO109I - PpuMI (597) DraIII (638) ScaI (1532) BstAPI (1612) BstEII (1645) SexAI* (1649) PciI (1768) AhdI (1769) PspOMI (2282) ApaI - BanII (2286) AccI (2358) PfoI* (2498) SpeI (2528) AleI (2711) pEZ™ BAC 7260 bp
SacII  (6773)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
FseI  (6769)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BciVI  (6758)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
MreI  (6758)
1 site
C G C C G G C G G C G G C C G C
XcmI  (6568)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
XbaI  (6058)
1 site
T C T A G A A G A T C T
EcoNI  (5784)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (5761)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (5759)
1 site
C C C G G G G G G C C C
XmaI  (5759)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
FspAI  (5502)
1 site
R T G C G C A Y Y A C G C G T R
BsrGI  (5470)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SbfI  (5233)
1 site
C C T G C A G G G G A C G T C C
BlpI  (4772)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AfeI  (4688)
1 site
A G C G C T T C G C G A
MfeI  (4263)
1 site
C A A T T G G T T A A C
NdeI  (4247)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstXI  (4173)
1 site
C C A N N N N N N T G G G G T N N N N N N A