pDNR-LIB

Donor vector for transferring a cloned cDNA into an acceptor vector using Cre recombinase in the Creator™ system.

Sequence Author: Clontech (TaKaRa)

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TaqII (4147) BmtI (4114) NheI (4110) BglII (4082) M13 fwd MluI (4059) BssSI - BssSαI (3831) BciVI (3806) PspFI (3704) BseYI (3700) ApaLI (3690) AlwNI (3595) BspHI (3284) AscI (3170) BfuAI - BspMI (3148) BstZ17I (2851) EarI (2486) StuI * (2481) MslI (2240) BsgI (2159) SalI (45) NdeI (61) TspMI - XmaI (66) SmaI (68) EcoRI (71) PstI - SbfI (93) MfeI (148) NmeAIII (285) PaeR7I - XhoI (311) HindIII (317) XbaI (323) BstXI (336) BmrI (344) PspOMI (346) ApaI (350) stop codons AvrII (384) SpeI (403) Eco53kI (517) SacI (519) ScaI (558) SspI (663) NcoI (670) PasI (740) PflMI * (746) BsmBI - Esp3I (747) BpmI (855) BsrDI (958) BspEI (975) ZraI (1262) AatII (1264) SacII (1645) BsaAI - SnaBI (1973) BsrGI (2038) pDNR-LIB 4161 bp
TaqII  (4147)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BmtI  (4114)
1 site
G C T A G C C G A T C G
NheI  (4110)
1 site
G C T A G C C G A T C G
BglII  (4082)
1 site
A G A T C T T C T A G A
MluI  (4059)
1 site
A C G C G T T G C G C A
BssSI  (3831)
1 site
C A C G A G G T G C T C
BssSαI  (3831)
1 site
C A C G A G G T G C T C
BciVI  (3806)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
PspFI  (3704)
1 site
C C C A G C G G G T C G
BseYI  (3700)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
ApaLI  (3690)
1 site
G T G C A C C A C G T G
AlwNI  (3595)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspHI  (3284)
1 site
T C A T G A A G T A C T
AscI  (3170)
1 site
G G C G C G C C C C G C G C G G
BfuAI  (3148)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3148)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BstZ17I  (2851)
1 site
G T A T A C C A T A T G
EarI  (2486)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
StuI  (2481)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
MslI  (2240)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsgI  (2159)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SalI  (45)
1 site
G T C G A C C A G C T G
NdeI  (61)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
TspMI  (66)
1 site
C C C G G G G G G C C C
XmaI  (66)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (68)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (71)
1 site
G A A T T C C T T A A G
PstI  (93)
1 site
C T G C A G G A C G T C
SbfI  (93)
1 site
C C T G C A G G G G A C G T C C
MfeI  (148)
1 site
C A A T T G G T T A A C
NmeAIII  (285)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PaeR7I  (311)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (311)
1 site
C T C G A G G A G C T C
HindIII  (317)
1 site
A A G C T T T T C G A A
XbaI  (323)
1 site
T C T A G A A G A T C T
BstXI  (336)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BmrI  (344)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
PspOMI  (346)
1 site
G G G C C C C C C G G G
ApaI  (350)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (384)
1 site
C C T A G G G G A T C C
SpeI  (403)
1 site
A C T A G T T G A T C A
Eco53kI  (517)
1 site
G A G C T C C T C G A G
SacI  (519)
1 site
G A G C T C C T C G A G
ScaI  (558)
1 site
A G T A C T T C A T G A
SspI  (663)
1 site
A A T A T T T T A T A A
NcoI  (670)
1 site
C C A T G G G G T A C C
PasI  (740)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (746)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BsmBI  (747)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (747)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BpmI  (855)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsrDI  (958)
1 site
G C A A T G N N C G