pEZ BAC (HpaI linearized)

HpaI-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic inserts.

Sequence Author: Lucigen

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SacII (6780) FseI (6776) BciVI - MreI (6765) XcmI (6575) XbaI (6065) EcoNI (5791) SmaI (5768) TspMI - XmaI (5766) FspAI (5509) BsrGI (5477) SbfI (5240) BlpI (4779) AfeI (4695) MfeI (4270) NdeI (4254) BstXI (4180) KpnI (4054) Acc65I (4050) PflFI - Tth111I (3989) BseRI (3563) BclI* (3459) T3Te terminatorAscI (7001) NotI (7029) BEZ-F1 (7119 .. 7142) BsrBI (7159) lac operatorHindIII (7236) SphI (7246) <HpaI> (7260) <HpaI> (0) BamHI (7) PmlI (19) EcoRI (24) BEZ-R1 (109 .. 132) KasI (211) NarI (212) SfoI (213) BbeI (215) NotI (241) PmeI (290) EcoO109I - PpuMI (604) DraIII (645) ScaI (1539) BstAPI (1619) BstEII (1652) SexAI* (1656) PciI (1775) AhdI (1776) PspOMI (2289) ApaI - BanII (2293) AccI (2365) PfoI* (2505) SpeI (2535) AleI (2718) pEZ™ BAC 7260 bp
SacII  (6780)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
FseI  (6776)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BciVI  (6765)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
MreI  (6765)
1 site
C G C C G G C G G C G G C C G C
XcmI  (6575)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
XbaI  (6065)
1 site
T C T A G A A G A T C T
EcoNI  (5791)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SmaI  (5768)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (5766)
1 site
C C C G G G G G G C C C
XmaI  (5766)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
FspAI  (5509)
1 site
R T G C G C A Y Y A C G C G T R
BsrGI  (5477)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SbfI  (5240)
1 site
C C T G C A G G G G A C G T C C
BlpI  (4779)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AfeI  (4695)
1 site
A G C G C T T C G C G A
MfeI  (4270)
1 site
C A A T T G G T T A A C
NdeI  (4254)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstXI  (4180)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C