M13mp19
M13 bacteriophage vector containing a multiple cloning site (MCS). The MCS is reversed relative to M13mp18.
Sequence Author: New England Biolabs
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Sticky ends from different BglI sites may not be compatible. |
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The 1-base overhangs produced by BmrI may be hard to ligate.Sticky ends from different BmrI sites may not be compatible.Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium. |
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SmaI can be used at 37°C for brief incubations. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Sticky ends from different BtsαI sites may not be compatible. |
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