pOSIP-KO
Prokaryotic one-step cloning and chromosomal integration vector encoding a kanamycin resistance marker and the phage 186 integrase.
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different PpuMI sites may not be compatible. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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ApaI can be used between 25°C and 37°C. |
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Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present. Sticky ends from different EarI sites may not be compatible. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different BstAPI sites may not be compatible. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Sticky ends from different AflIII sites may not be compatible. |
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