CMV promoter

Human cytomegalovirus (CMV) immediate early enhancer and promoter.
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No matches
500 400 300 200 100 End (508) EciI (436) BanI (405) MslI (305) BtgI - StyI - NcoI (300) BsaAI - SnaBI (280) BtgZI (274) BmrI (229) NdeI (174) Start (0) CMV enhancer CMV promoter CMV promoter 508 bp
End  (508)
0 sites
EciI  (436)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BanI  (405)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
MslI  (305)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BtgI  (300)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
StyI  (300)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (300)
1 site
C C A T G G G G T A C C
BsaAI  (280)
1 site
Y A C G T R R T G C A Y
SnaBI  (280)
1 site
T A C G T A A T G C A T
BtgZI  (274)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BmrI  (229)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
NdeI  (174)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
Start  (0)
0 sites
CMV enhancer
1 .. 304  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1 .. 304  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
305 .. 508  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
305 .. 508  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
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Download CMV promoter.dna file

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Individual Sequences & Maps

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