GAL1 promoter

Yeast promoter with upstream activating sequence mediating Gal4-dependent induction.
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
400 300 200 100 End (442) BstAPI (322) BtgZI (296) AseI (269) EarI (152) BtsI - BtsαI (108) BsiEI (75) AgeI - BsrFI - BsaWI (70) NmeAIII (39) BseRI (38) BsrBI (19) Start (0) GAL1 promoter UAS GAL1 promoter 442 bp
End  (442)
0 sites
BstAPI  (322)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BtgZI  (296)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AseI  (269)
1 site
A T T A A T T A A T T A
EarI  (152)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BtsI  (108)
1 site
G C A G T G N N C G T C A C
BtsαI  (108)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BsiEI  (75)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
AgeI  (70)
1 site
A C C G G T T G G C C A
BsrFI  (70)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaWI  (70)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
NmeAIII  (39)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BseRI  (38)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsrBI  (19)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Start  (0)
0 sites
GAL1 promoter
1 .. 442  =  442 bp
inducible promoter, regulated by Gal4
GAL1 promoter
1 .. 442  =  442 bp
inducible promoter, regulated by Gal4
UAS
1 .. 118  =  118 bp
upstream activating sequence mediating Gal4-dependent induction
UAS
1 .. 118  =  118 bp
upstream activating sequence mediating Gal4-dependent induction
Click here to try SnapGene

Download GAL1 promoter.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.