pE-SUMOpro Kan

Bacterial vector with a kanamycin resistance marker, for regulated expression of recombinant proteins with a cleavable 6xHis-SUMO tag.

Sequence Author: LifeSensors

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SgrAI (5588) SphI (5440) BstAPI (5231) MluI (4907) BstEII (4725) NmeAIII (4707) ApaI (4704) PspOMI (4700) BssHII (4496) EcoRV (4461) HpaI (4405) PshAI (4066) BglI (3850) FspI - FspAI (3829) PflFI - Tth111I (3064) BstZ17I (3039) TatI (3002) BspQI - SapI (2923) T7 promoter XbaI (67) RBS NcoI (106) ATG 6xHis KflI (133) PstI - SbfI (142) DraI (214) AflII (323) BsaI (427) SpeI (439) Acc65I (445) AgeI (448) KpnI (449) BsaI (457) BamHI (463) Eco53kI (477) SacI (479) SalI (482) HindIII (488) EagI - NotI (495) PaeR7I - PspXI - XhoI (503) 6xHis DraIII (910) PsiI (1035) AsiSI - PvuI (1610) TspMI - XmaI (1732) SmaI (1734) BspDI - ClaI (1915) NruI (1951) AlwNI (2397) BssSαI (2633) PciI (2806) pE-SUMOpro Kan 5628 bp
SgrAI  (5588)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (5440)
1 site
G C A T G C C G T A C G
BstAPI  (5231)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MluI  (4907)
1 site
A C G C G T T G C G C A
BstEII  (4725)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (4707)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (4704)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (4700)
1 site
G G G C C C C C C G G G
BssHII  (4496)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoRV  (4461)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HpaI  (4405)
1 site
G T T A A C C A A T T G
PshAI  (4066)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BglI  (3850)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (3829)
1 site
T G C G C A A C G C G T
FspAI  (3829)
1 site
R T G C G C A Y Y A C G C G T R
PflFI  (3064)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3064)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstZ17I  (3039)
1 site
G T A T A C C A T A T G
TatI  (3002)
1 site
W G T A C W W C A T G W
BspQI  (2923)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2923)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
XbaI  (67)
1 site
T C T A G A A G A T C T
NcoI  (106)
1 site
C C A T G G G G T A C C
KflI  (133)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PstI  (142)
1 site
C T G C A G G A C G T C
SbfI  (142)
1 site
C C T G C A G G G G A C G T C C
DraI  (214)
1 site
T T T A A A A A A T T T
AflII  (323)
1 site
C T T A A G G A A T T C
BsaI  (427)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
SpeI  (439)
1 site
A C T A G T T G A T C A
Acc65I  (445)
1 site
G G T A C C C C A T G G
AgeI  (448)
1 site
A C C G G T T G G C C A
KpnI  (449)
1 site
G G T A C C C C A T G G
BsaI  (457)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BamHI  (463)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Eco53kI  (477)
1 site
G A G C T C C T C G A G
SacI  (479)
1 site
G A G C T C C T C G A G
SalI  (482)
1 site
G T C G A C C A G C T G
HindIII  (488)
1 site
A A G C T T T T C G A A
EagI  (495)
1 site
C G G C C G G C C G G C
NotI  (495)
1 site
G C G G C C G C C G C C G G C G
PaeR7I  (503)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (503)
1 site
V C T C G A G B B G A G C T C V
XhoI  (503)
1 site
C T C G A G G A G C T C
DraIII  (910)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (1035)
1 site
T T A T A A A A T A T T
AsiSI  (1610)
1 site
G C G A T C G C C G C T A G C G
PvuI  (1610)
1 site
C G A T C G G C T A G C
TspMI  (1732)
1 site
C C C G G G G G G C C C
XmaI  (1732)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1734)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BspDI  (1915)
1 site
A T C G A T T A G C T A
ClaI  (1915)
1 site
A T C G A T T A G C T A
NruI  (1951)
1 site
T C G C G A A G C G C T
AlwNI  (2397)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (2633)
1 site
C A C G A G G T G C T C
PciI  (2806)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
lacI
4180 .. 5262  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
4180 .. 5262  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
KanR
1225 .. 2040  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
1225 .. 2040  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
ori
2162 .. 2750  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2162 .. 2750  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
677 .. 1132  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
677 .. 1132  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
ATG
108 .. 110  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
108 .. 110  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
114 .. 131  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
114 .. 131  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
SUMO
144 .. 431  =  288 bp
96 amino acids  =  11.0 kDa
Product: cleavable ubiquitin-like protein tag
SUMO
144 .. 431  =  288 bp
96 amino acids  =  11.0 kDa
Product: cleavable ubiquitin-like protein tag
rop
3180 .. 3371  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
rop
3180 .. 3371  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
lacI promoter
5263 .. 5340  =  78 bp
lacI promoter
5263 .. 5340  =  78 bp
MCS
439 .. 508  =  70 bp
multiple cloning site
MCS
439 .. 508  =  70 bp
multiple cloning site
T7 terminator
593 .. 640  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
593 .. 640  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
40 .. 64  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
40 .. 64  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RBS
79 .. 101  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
RBS
79 .. 101  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
509 .. 526  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
509 .. 526  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ORF:  3403 .. 3771  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  5438 .. 49  =  240 bp
ORF:  79 amino acids  =  8.0 kDa
ORF:  108 .. 434  =  327 bp
ORF:  108 amino acids  =  12.4 kDa
ORF:  4146 .. 4397  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  5301 .. 5564  =  264 bp
ORF:  87 amino acids  =  9.5 kDa
ORF:  1225 .. 2040  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
ORF:  4180 .. 5139  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  3180 .. 3404  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  3768 .. 4124  =  357 bp
ORF:  118 amino acids  =  13.0 kDa
ORF:  4160 .. 4423  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  5012 .. 5512  =  501 bp
ORF:  166 amino acids  =  17.5 kDa
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