pICOz

Minimal 1185-bp cloning vector derived from pUC18, with a Zeocin™ resistance marker. See also pUCmu.

Sequence Author: BCCM/GeneCorner Plasmid Collection

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MluI (1) BspHI (1169) SspI (1138) EarI (1129) BssHII - MauBI (1080) AatII (1078) ZraI (1076) SgrAI (1002) BmgBI (986) CsiI - SexAI * (952) FseI (848) BglI (818) EagI (785) DraIII (770) BseRI (747) AcuI - Eco57MI (621) NruI (8) NdeI (16) HpaI (24) NcoI - StyI (28) HindIII (36) BfuAI - BspMI (41) SphI (46) PstI - SbfI (52) SalI (54) AccI (55) XbaI (60) BamHI (66) Acc65I (74) KpnI (78) Eco53kI (82) SacI (84) ApoI - EcoRI (86) EcoRV (95) PaeR7I - XhoI (99) SpeI (105) PspOMI (111) ApaI (115) DraI - PmeI (120) PciI (125) DrdI (181) BssSI - BssSαI (246) HaeII (321) BseYI (377) PspFI (381) AlwNI (489) pICOz 1185 bp
MluI  (1)
1 site
A C G C G T T G C G C A
BspHI  (1169)
1 site
T C A T G A A G T A C T
SspI  (1138)
1 site
A A T A T T T T A T A A
EarI  (1129)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BssHII  (1080)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (1080)
1 site
C G C G C G C G G C G C G C G C
AatII  (1078)
1 site
G A C G T C C T G C A G
ZraI  (1076)
1 site
G A C G T C C T G C A G
SgrAI  (1002)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BmgBI  (986)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
CsiI  (952)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (952)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
FseI  (848)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BglI  (818)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
EagI  (785)
1 site
C G G C C G G C C G G C
DraIII  (770)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BseRI  (747)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AcuI  (621)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (621)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NruI  (8)
1 site
T C G C G A A G C G C T
NdeI  (16)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
HpaI  (24)
1 site
G T T A A C C A A T T G
NcoI  (28)
1 site
C C A T G G G G T A C C
StyI  (28)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
HindIII  (36)
1 site
A A G C T T T T C G A A
BfuAI  (41)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (41)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (46)
1 site
G C A T G C C G T A C G
PstI  (52)
1 site
C T G C A G G A C G T C
SbfI  (52)
1 site
C C T G C A G G G G A C G T C C
SalI  (54)
1 site
G T C G A C C A G C T G
AccI  (55)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
XbaI  (60)
1 site
T C T A G A A G A T C T
BamHI  (66)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (74)
1 site
G G T A C C C C A T G G
KpnI  (78)
1 site
G G T A C C C C A T G G
Eco53kI  (82)
1 site
G A G C T C C T C G A G
SacI  (84)
1 site
G A G C T C C T C G A G
ApoI  (86)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (86)
1 site
G A A T T C C T T A A G
EcoRV  (95)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaeR7I  (99)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (99)
1 site
C T C G A G G A G C T C
SpeI  (105)
1 site
A C T A G T T G A T C A
PspOMI  (111)
1 site
G G G C C C C C C G G G
ApaI  (115)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
DraI  (120)
1 site
T T T A A A A A A T T T
PmeI  (120)
1 site
G T T T A A A C C A A A T T T G
PciI  (125)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
DrdI  (181)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BssSI  (246)
1 site
C A C G A G G T G C T C
BssSαI  (246)
1 site
C A C G A G G T G C T C
HaeII  (321)
1 site
R G C G C Y Y C G C G R
BseYI  (377)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (381)
1 site
C C C A G C G G G T C G
AlwNI  (489)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ori
134 .. 722  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
134 .. 722  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BleoR
747 .. 1121  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
747 .. 1121  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
MCS
1 .. 130  =  130 bp
multiple cloning site
MCS
1 .. 130  =  130 bp
multiple cloning site
AmpR promoter
1122 .. 1185  =  64 bp
AmpR promoter
1122 .. 1185  =  64 bp
ORF:  841 .. 1146  =  306 bp
ORF:  101 amino acids  =  11.0 kDa
ORF:  1171 .. 459  =  474 bp
ORF:  157 amino acids  =  17.6 kDa
ORF:  742 .. 45  =  489 bp
ORF:  162 amino acids  =  17.6 kDa
ORF:  747 .. 1121  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
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